{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:08:16Z","timestamp":1791504496800,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study explores novel therapeutic avenues for diabetes, a global health concern marked by elevated blood glucose levels. We investigated the anti\u2010diabetic potential of\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Gymnema Sylvestre<\/jats:styled-content>\n                      's\n                    <\/jats:italic>\n                    bioactive compounds, including Gymnemic acid I, Stigmasterol, Deacylgymnemic acid, Beta\u2010Amyrin acetate, Longispinogenin, Gymnemic acid II, Gymnemic acid, Gymnemic acid X, Gymnemaside VI, Phytic acid and Gymnemic acid X. Employing network pharmacology, molecular docking and molecular dynamics (MD), we elucidated the potential mechanism of action. SwissTargetPrediction identified targets for bioactive constituents, while DisGeNET provided diabetes\u2010related targets. A GeneVenn diagram revealed 397 common potential targets for diabetes management. The protein\u2013protein interaction network, constructed via the STRING database, underwent topological analysis in Cytoscape, identifying AKT1, SRC, TNF, PPARG and IL1B as top targets. Gene ontology analysis using FunRich identified crucial roles of screened targets in integrin family cell surface interactions and glypican pathways for diabetes management. Molecular interactions and binding affinities with the top target, AKT1, were assessed, with Gymnemic acid I displaying the least binding energy (\u22129.813) with H\u2010 and non\u2010H\u2010bond interactions. Molecular dynamics simulations provided insights into the distinct behaviours of Gymnemic acid I within the protein complex. In conclusion, our study elucidates the potential anti\u2010diabetic mechanism of Gymnemic acid I, underscoring the need for further in\u00a0vitro, in\u00a0vivo and clinical studies to validate our findings.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70349","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T02:23:33Z","timestamp":1736907813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Deciphering the Anti\u2010Diabetic Potential of\n                    <i>Gymnema Sylvestre<\/i>\n                    Using Integrated Computer\u2010Aided Drug Design and Network Pharmacology"],"prefix":"10.1111","volume":"29","author":[{"given":"Amal","family":"Mayyas","sequence":"first","affiliation":[{"name":"Faculty of Health Sciences, Department of Pharmacy American University of Madaba  Madaba Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Al\u2010Samydai","sequence":"additional","affiliation":[{"name":"Pharmacological and Diagnostic Research Centre (PDRC), Faculty of Pharmacy Al\u2010Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amjad Ibrahim","family":"Oraibi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy AL\u2010Manara College for Medical Sciences  Amarah Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawres","family":"Debbabi","sequence":"additional","affiliation":[{"name":"Research Laboratory for Bioactive Natural Products and Biotechnology LR24ES14, Faculty of Dental Medicine of Monastir University of Monastir  Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara S.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Hilla University College  Babylon Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany Aqeel","family":"Al\u2010Hussainy","sequence":"additional","affiliation":[{"name":"Al\u2010Nisour University College, Pharmacy Department  Baghdad Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Mohammad","family":"Salamatullah","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Nutrition, College of Food and Agricultural Sciences King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0685-8243","authenticated-orcid":false,"given":"Musaab","family":"Dauelbait","sequence":"additional","affiliation":[{"name":"Department of Scientific Translation, Faculty of Translation University of Bahri  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3707-8461","authenticated-orcid":false,"given":"Mohammed","family":"Bourhia","sequence":"additional","affiliation":[{"name":"Swalife Biotech Ltd Unit 3D North Point House North Point Business Park  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid S.","family":"Almaary","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(02)00059-4"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/830285","article-title":"Phytochemical and Pharmacological Properties of \n                     Gymnema sylvestre\n                  : An Important Medicinal Plant","volume":"2014","author":"Tiwari P.","year":"2014","journal-title":"BioMed Research International"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijo.IJO_1237_21"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.104998"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40200-020-00628-8"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(15)30173-8"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27072175"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12010118"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42826-021-00101-4"},{"key":"e_1_2_11_11_1","first-page":"1","article-title":"Bioactive Compounds in Anti\u2010Diabetic Plants: From Herbal Medicine to Modern Drug Discovery","volume":"9","author":"Tran N.","year":"2020","journal-title":"Biology (Basel)"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920101606150407112903"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01223"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114908"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6458"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/B978-008045382-8.00077-0","article-title":"Natural Products as Sweeteners and Sweetness Modifiers","volume":"3","author":"Kinghorn A. D.","year":"2010","journal-title":"Comprehensive Natural Products II: Chemistry and Biology"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11101912"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.2513"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(90)90146-A"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327116638727"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116306"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-27540-4"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-022-03662-6"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5561129"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.30430"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c00156"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcs186"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku293"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.6026\/97320630001420"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg034"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm051256o"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(96)00103-7"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/ct900587b"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.8b01026"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00214a001"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.862164"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.27173"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27621"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T00:47:58Z","timestamp":1746838078000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/jcmm.70349"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70349","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70349"}}