{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T08:37:11Z","timestamp":1775032631810,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Trophoblast glycoprotein (TPBG) plays a significant part in the growth of specific cancers, yet its connection to gastric cancer (GC) remains uncertain. This research seeks to analyse the fluctuation in TPBG levels in GC and evaluate how TPBG expression relates to the prognosis of GC patients. TPBG expression in GC and normal gastric tissues was investigated in The Cancer Genome Atlas (TCGA) and Genotype\u2010Tissue Expression (GTEx) database, further extracting the immunohistochemistry images from HPA database and validating by Western blot. The connection between TPBG and GC patients' survival rates was investigated by Kaplan\u2013Meier and COX regression analysis. Genes related to TPBG were enriched using GO and KEGG data. In\u00a0vitro and in\u00a0vivo tumour models were utilised to evaluate the function of TPBG in GC. Western blot analysis was performed to detect the expression of PI3K\/AKT signalling pathway proteins following TPBG knockdown. Immune infiltration was analysed using the CIBERSOFT and ssGSEA methods. The association between TPBG and immune cells that infiltrate tumours was evaluated through the utilisation of GSVA. TPBG expression increased in several tumour tissues (including GC) more than in adjacent noncancerous tissues. Elevated TPBG level predicted worse outcomes, such as poorer overall survival, pathological stage, and therapy response in GC. Enrichment analysis primarily focused on biological processes like the organisation of external encapsulating structures, extracellular structure, and collagen metabolism. Biological experiments further demonstrated that TPBG knockdown successfully inhibits the progression, migration, and invasion of GC cells. Western blot analysis revealed that TPBG knockdown inhibits the PI3K\/AKT signalling pathway. Furthermore, TPBG is associated with the infiltration of immune cells in GC, which correlates with the expression of macrophage cells. There is a positive relationship between TPBG and malignant behaviour of GC tissues and cells, suggesting that TPBG can be useful for diagnosing and prognosing GC.<\/jats:p>","DOI":"10.1111\/jcmm.70354","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T17:35:22Z","timestamp":1737135322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In\u2010Depth Examination of <scp>TPBG<\/scp> as a New Predictive Indicator for Gastric Cancer"],"prefix":"10.1111","volume":"29","author":[{"given":"Lianlei","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology The First People's Hospital of Linping District  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyan","family":"Weng","sequence":"additional","affiliation":[{"name":"The First Clinical Medical of Zhejiang Chinese Medical University  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Affiliated Hospital of Hangzhou Normal University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Affiliated Hospital of Hangzhou Normal University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kexin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Affiliated Hospital of Hangzhou Normal University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guodong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Affiliated Hospital of Hangzhou Normal University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqing","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Affiliated Hospital of Hangzhou Normal University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4192-5766","authenticated-orcid":false,"given":"Chenghai","family":"He","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Affiliated Hospital of Hangzhou Normal University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(20)31288\u20105"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262\u2010016\u20101917\u20103"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.Ccr\u201016\u20101834"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2014.07.004"},{"key":"e_1_2_10_7_1","first-page":"99","article-title":"5T4 Oncotrophoblast Glycoprotein: Janus Molecule in Life and a Novel Potential Target Against Tumors","volume":"4","author":"Zhao Y.","year":"2007","journal-title":"Cellular & Molecular Immunology"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.Cir\u201013\u20100035"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz210"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1994.173"},{"key":"e_1_2_10_12_1","first-page":"1923","article-title":"Low Intercellular Adhesion Molecule 1 and High 5T4 Expression on Tumor Cells Correlate With Reduced Disease\u2010Free Survival in Colorectal Carcinoma Patients","volume":"3","author":"Mulder W. 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