{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:58:56Z","timestamp":1792713536996,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82200434"],"award-info":[{"award-number":["82200434"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bisphenol A (BPA) is a widely used industrial chemical with potential endocrine\u2010disrupting effects on metabolic processes. This study investigates the impact of BPA on hepatic function and transcriptional regulation in mouse livers and AML12 cells. Male mice were exposed to low (5\u2009g\/kg) and high (50\u2009g\/kg) doses of BPA for six weeks. Transcriptomic analysis was performed on liver tissues, and histological examinations were conducted. AML12 cells were treated with varying BPA concentrations, and PPARG transcriptional activity was assessed using a luciferase reporter assay. Additionally, molecular docking, molecular dynamics (MD) simulations, drug affinity responsive target stability (DARTS), cellular thermal shift assay (CETSA), MM\u2010PBSA calculations, and multi\u2010species protein structure comparative analysis were employed to analyse the interaction between BPA and PPARG. Transcriptomic analysis revealed a decrease in differentially expressed genes with higher BPA doses, with low\u2010dose exposure significantly downregulating hepatic\n                    <jats:italic>Cpt1a<\/jats:italic>\n                    mRNA levels. Histological examination indicated lipid vacuole formation at high doses without collagen deposition. BPA consistently inhibited PPARG activity in both MCF7 cells and mouse livers. BPA exposure disrupts hepatic lipid metabolism and PPARG activity, highlighting its role as an endocrine disruptor. Further research is needed to elucidate the long\u2010term effects of BPA on liver health.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70416","type":"journal-article","created":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T00:21:08Z","timestamp":1746836468000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Disruption of\n                    <scp>PPARG<\/scp>\n                    Activity and\n                    <scp>CPT1A<\/scp>\n                    Regulation by Bisphenol A: Implications for Hepatic Lipid Metabolism"],"prefix":"10.1111","volume":"29","author":[{"given":"Xiliang","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery, Central China Subcenter of National Center for Cardiovascular Diseases, Henan Cardiovascular Disease Center, Fuwai Central\u2010China Cardiovascular Hospital Central China Fuwai Hospital of Zhengzhou University  Zhengzhou 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Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research Southwest Medical University  Luzhou 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