{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T14:35:47Z","timestamp":1782830147315,"version":"3.54.5"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800811"],"award-info":[{"award-number":["81800811"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study investigates the differences in ligand\u2013receptor interactions between the communication network of vascular endothelial cells (ECs) and photoreceptor cells (PRCs)in diabetic retinopathy (DR) the mechanism was verified by animal experiments. The GSE209872 data set, including retinal specimens from five Sprague\u2013Dawley rats induced by streptozotocin, was obtained from Gene Expression Omnibus. CM and EC data were extracted individually for reclustering, functional enrichment and trajectory analyses. Cell communication analysis was conducted to investigate the altered signals and significant ligand\u2013receptor interactions. Moreover, novel ligand\u2013receptor interactions were validated using immunofluorescence staining using 2, 4 and 8\u2009weeks DR model; DR was treated with AAV\u2010shANGPTL4, and retinal function was detected by Haematoxylin and eosin staining (HE), TUNEL and ELISA. The expression of ligand\u2013receptor in DR Retina was detected by qPCR and immunohistochemistry. Nine cell types were determined in DR. Cellular communication results revealed four signalling pathways, including PTN, MK, ANGPTL and CXCL, that were significantly changed in DR. Furthermore, 3 ligand\u2010receptor pairs (Ptn\u2010Ncl, Mkd\u2010Ncl and Angptl4\u2010Sdc4) were obviously upregulated between ECs and PRCs, the expression of which was verified via immunofluorescence in the DR model. After treatment with AAV\u2010shANGPTL4, the retinal thickness and average density of RGCs were decreased (<jats:italic>p<\/jats:italic> &lt;\u20090.05). TUNEL staining showed that knocking down ANGPTL4 reduced the apoptosis of DR (<jats:italic>p<\/jats:italic> &lt;\u20090.05), and VEGF and IGF\u20101 expression were downregulated (<jats:italic>p<\/jats:italic> &lt;\u20090.01). The expression of ligand\u2013receptors also decreased in the DR Model (<jats:italic>p<\/jats:italic> &lt;\u20090.01). The vascular ECs and PRCs demonstrate significant heterogeneities in DR. ANGPTL4 was a decreased ligand\u2013receptor expression and improved retinal function as a potential therapeutic target against DR.<\/jats:p>","DOI":"10.1111\/jcmm.70442","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T20:53:33Z","timestamp":1741726413000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Exploration of Disturbance of Capillary and Photoreceptor Communication Networks in Diabetic Retinopathy Through Single\u2010Cell RNA\u2010Seq"],"prefix":"10.1111","volume":"29","author":[{"given":"Ning","family":"Wang","sequence":"first","affiliation":[{"name":"Eye Hospital, The First Affiliated Hospital Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huibo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Affiliated Hospital Harbin Medical University  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