{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T18:51:09Z","timestamp":1788202269757,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Effective therapy against <jats:styled-content style=\"fixed-case\"><jats:italic>Helicobacter pylori<\/jats:italic><\/jats:styled-content> hinges on a timely and accurate diagnosis. The objective is to assess <jats:styled-content style=\"fixed-case\"><jats:italic>H.\u2009pylori<\/jats:italic><\/jats:styled-content> infection in dyspeptic patients and compare various indicative tests. After approval, gastrointestinal biopsies and blood samples of 96 subjects exhibiting gastroduodenal symptoms were collected; both invasive and non\u2010invasive tests were employed to analyse the samples. Results revealed 40 cases (41.67%) positive for <jats:styled-content style=\"fixed-case\"><jats:italic>H.\u2009pylori<\/jats:italic><\/jats:styled-content> via histopathology and rapid urease testing, while 46 subjects tested positive for IgA and IgG antibodies via ELISA. Eighteen biopsies showed positivity in the culture test, corroborated by endoscopic examination and biochemical assessments (urease, catalase and oxidase). The isolates showed various degrees of resistance to antibiotics, while polymyxin B showed the highest (100%) followed by amoxicillin (88.90%) and kanamycin (77.78%). Additionally, the <jats:italic>CagA<\/jats:italic> gene presence was detected in 18 individuals through molecular methods. Sensitivity and specificity percentages (%) varied among diagnostic methods: histopathology (95\/77), rapid urease (100\/83.5), gram staining (85.7\/90), IgG serology (100\/66.6), IgA serology (100\/79.5), PCR (100\/75), RUT and IgG serology combination (100\/79.04), and RUT, Gram staining and IgG serology combination (100\/92.4), respectively. PCR emerged as the most reliable test. In the current investigation, other tests also exhibited high sensitivity and specificity values. Thus, employing comparative detection methods rather than relying solely on one methodology is advisable for accurate detection.<\/jats:p>","DOI":"10.1111\/jcmm.70487","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T14:33:49Z","timestamp":1742394829000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative Analysis of Diagnostic Techniques for <i>Helicobacter pylori<\/i> Infection: Insights for Effective Therapy"],"prefix":"10.1111","volume":"29","author":[{"given":"Ahmed","family":"Mujtaba","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, Faculty of Engineering Sciences and Technology Hamdard University Islamabad Campus  Islamabad Pakistan"},{"name":"Institute of Food and Nutritional Sciences PMAS\u2010Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Suhail","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Institute of Food and Nutritional Sciences PMAS\u2010Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Parveen","sequence":"additional","affiliation":[{"name":"Institute of Food and Nutritional Sciences PMAS\u2010Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noreen","family":"Sarwar","sequence":"additional","affiliation":[{"name":"Institute of Microbiology University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suliman A.","family":"Alsagaby","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Applied Medical Sciences Majmaah University  AL\u2010Majmaah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ahsan","family":"Raza","sequence":"additional","affiliation":[{"name":"Department of Zoology Govt. Graduate College for Boys  Gujranwala Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Abdelgawad","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy Jouf University  Sakaka Aljouf Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed M.","family":"Ghoneim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice, College of Pharmacy AlMaarefa University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed H.","family":"El\u2010Ghorab","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science Jouf University  Sakaka Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samy","family":"Selim","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences Jouf University  Sakaka Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleed","family":"Al Abdulmonem","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Medicine Qassim University  Buraidah Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muzzamal","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Food Science Government College University Faisalabad  Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9241-0266","authenticated-orcid":false,"given":"Tadesse","family":"Fenta Yehuala","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Food Engineering, Bahir Dar Institute of Technology Bahir Dar University  Bahir Dar City Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.jcmgh.2021.02.013","article-title":"Helicobacter pylori Induced Rev\u2010Erb\u03b1 Foster Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense","volume":"12","author":"Mao F. 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I.","year":"2014","journal-title":"World Journal of Gastroenterology: WJG"},{"issue":"1","key":"e_1_2_10_36_1","first-page":"1","article-title":"Diagnosis of Helicobacter pylori Using the Rapid Urease Test","volume":"3","author":"Uotani T.","year":"2015","journal-title":"Annals of Translational Medicine"},{"issue":"12","key":"e_1_2_10_37_1","doi-asserted-by":"crossref","DOI":"10.1002\/14651858.CD008337.pub2","article-title":"Optimum Duration of Regimens for Helicobacter pylori Eradication","volume":"2013","author":"Yuan Y.","year":"2013","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"e_1_2_10_38_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2011\/940650","article-title":"Reliability of Diagnostic Tests for \n                     Helicobacter pylori\n                   Infection","volume":"2011","author":"Red\u00e9en S.","year":"2011","journal-title":"Gastroenterology Research and Practice"},{"issue":"3","key":"e_1_2_10_39_1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.32007\/jfacmedbagdad.533836","article-title":"The Role of ELISA Test in the Diagnosis of Helicobacter pylori Infection","volume":"53","author":"Mohsun N. 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J.","year":"1999","journal-title":"Journal of Clinical Microbiology"},{"issue":"5","key":"e_1_2_10_47_1","first-page":"1","article-title":"Advances in the Application of Molecular Diagnostic Techniques for the Detection of Infectious Disease Pathogens","volume":"27","author":"Liu Q.","year":"2023","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_10_48_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1590\/S0036-46652014000300003","article-title":"Resistance to Amoxicillin, Clarithromycin and Ciprofloxacin of \n                     Helicobacter pylori\n                   Isolated From Southern Brazil Patients","volume":"56","author":"Picoli S. U.","year":"2014","journal-title":"Revista Do Instituto de Medicina Tropical de S\u00e3o Paulo"},{"issue":"4","key":"e_1_2_10_49_1","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1177\/1559827617700824","article-title":"The Role of Antibiotic Stewardship in Promoting Appropriate Antibiotic Use","volume":"13","author":"Ha D. R.","year":"2019","journal-title":"American Journal of Lifestyle Medicine"},{"issue":"2","key":"e_1_2_10_50_1","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1590\/S1517-83822011000200005","article-title":"Isolation of Helicobacter pylori in Gastric Mucosa and Susceptibility to Five Antimicrobial Drugs in Southern Chile","volume":"42","author":"Otth L.","year":"2011","journal-title":"Brazilian Journal of Microbiology"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70487","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T05:10:35Z","timestamp":1743397835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70487"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/jcmm.70487"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70487","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70487"}}