{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T19:11:58Z","timestamp":1782587518486,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T00:00:00Z","timestamp":1742688000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing Municipality","doi-asserted-by":"publisher","award":["cstc2021jcyj\u2010msxmX0205"],"award-info":[{"award-number":["cstc2021jcyj\u2010msxmX0205"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Intervertebral Disc Degeneration (IDD) is a multifactorial result contributing to Low Back Pain (LBP) while Cartilage Intermediate Layer Protein\u20101 (CILP\u20101) is gradually up\u2010regulated along with IDD. Whether CILP\u20101 acts in a direct role promoting IDD via regulating matrix metabolism remains to be elucidated. Herein, we firstly detected the expression level of matrix\u2010related phenotypes in nucleus pulposus (NP) cells treated with CILP\u20101, including ADAMTS, MMPs, IL\u20106, Collagens, Aggrecan (ACAN) and SOX9. Meanwhile, the phosphorylation levels of MAPKs and NF\u2010\u03baB were detected to explore the involved signalling pathways, which were further validated by inhibition experiments. Furthermore, molecular docking analysis was employed to evaluate the possibility of CD47 acting as the direct receptor mediating CILP's regulation above, which was further validated by immunoprecipitation and inhibition experiment. Our findings have made a comprehensive investigation into the regulatory effect of CILP\u20101 on the matrix metabolism of NP cells and explored the underlying mechanism.<\/jats:p>","DOI":"10.1111\/jcmm.70506","type":"journal-article","created":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T03:39:42Z","timestamp":1742787582000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cartilage Intermediate Layer Protein\u20101 Promotes Extracellular Matrix Degeneration via Interacting With <scp>CD47<\/scp>"],"prefix":"10.1111","volume":"29","author":[{"given":"Jiezhong","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Orthopedics Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zehua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0164-9472","authenticated-orcid":false,"given":"Jinyue","family":"He","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2023.2186099"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22031080"},{"issue":"8","key":"e_1_2_12_4_1","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.1016\/j.ymthe.2023.06.010","article-title":"Smoking and Tetramer Tryptase Accelerate Intervertebral Disc Degeneration by Inducing METTL14\u2010Mediated DIXDC1 m(6) Modification","volume":"31","author":"Tu J.","year":"2023","journal-title":"Molecular Therapy"},{"key":"e_1_2_12_5_1","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.740200","article-title":"Causal Associations of Obesity With the Intervertebral Degeneration, Low Back Pain, and Sciatica: A Two\u2010Sample Mendelian Randomization Study","volume":"12","author":"Zhou J.","year":"2021","journal-title":"Frontiers in Endocrinology"},{"issue":"1","key":"e_1_2_12_6_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.3390\/ijms24010208","article-title":"Discogenic Low Back Pain: Anatomy, Pathophysiology and Treatments of Intervertebral Disc Degeneration","volume":"24","author":"Mohd Isa I. L.","year":"2022","journal-title":"International Journal of Molecular Sciences"},{"issue":"11","key":"e_1_2_12_7_1","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1002\/jor.24639","article-title":"Inflammatory Cytokine and Catabolic Enzyme Expression in a Goat Model of Intervertebral Disc Degeneration","volume":"38","author":"Zhang C.","year":"2020","journal-title":"Journal of Orthopaedic Research"},{"key":"e_1_2_12_8_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.jbiomech.2017.04.027","article-title":"Are Axial Intervertebral Disc Biomechanics Determined by Osmosis?","volume":"70","author":"Vergroesen P. A.","year":"2018","journal-title":"Journal of Biomechanics"},{"issue":"11","key":"e_1_2_12_9_1","doi-asserted-by":"crossref","first-page":"3338","DOI":"10.1080\/15548627.2021.1872227","article-title":"HIF1A Alleviates Compression\u2010Induced Apoptosis of Nucleus Pulposus Derived Stem Cells via Upregulating Autophagy","volume":"17","author":"He R.","year":"2021","journal-title":"Autophagy"},{"issue":"2","key":"e_1_2_12_10_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.3892\/ijmm.2020.4832","article-title":"Cartilage Intermediate Layer Protein Affects the Progression of Intervertebral Disc Degeneration by Regulating the Extracellular Microenvironment (Review)","volume":"47","author":"Liu L.","year":"2021","journal-title":"International Journal of Molecular Medicine"},{"issue":"4","key":"e_1_2_12_11_1","doi-asserted-by":"crossref","first-page":"E203","DOI":"10.1097\/BRS.0b013e31822dcf47","article-title":"Cartilage Intermediate Layer Protein (CILP) Regulation in Intervertebral Discs. The Effect of Age, Degeneration, and Bone Morphogenetic Protein\u20102","volume":"37","author":"Wang Z.","year":"2012","journal-title":"Spine (Phila Pa 1976)"},{"key":"e_1_2_12_12_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.yjmcc.2018.02.006","article-title":"Cartilage Intermediate Layer Protein\u20101 Alleviates Pressure Overload\u2010Induced Cardiac Fibrosis via Interfering TGF\u2010beta1 Signaling","volume":"116","author":"Zhang C. 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