{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:32:54Z","timestamp":1772994774499,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100030782","name":"Promex Stiftung fur die Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100030782","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Valve interstitial cells (VICs) play a critical role in aortic valve calcification and angiogenic processes associated with calcific aortic valve stenosis (CAVS). Within the same valve, VICs from differently calcified regions can exhibit diverse phenotypic and functional properties. We hypothesised that VICs isolated from noncalcified (NC\u2010VICs) and calcified (C\u2010VICs) areas of human aortic valves possess distinct angiogenic characteristics. In this study, we isolated C\u2010VICs and NC\u2010VICs from 23 valves obtained after aortic valve replacement due to CAVS. Both VIC types exhibited similar phenotypes in culture, characterised by morphology, expression of mesenchymal\/fibroblastic markers, proliferation and osteogenic differentiation. No significant differences were observed in the secretion of angiogenic factors, including VEGF\u2010A, Ang\u20101, Ang\u20102, PlGF, bFGF between NC\u2010VICs and C\u2010VICs. However, when co\u2010injected with endothelial colony\u2010forming cells (ECFCs) into Matrigel implants in\u00a0vivo in mice, implants containing NC\u2010VICs showed significantly higher microvessel density compared to those with C\u2010VICs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Additionally, NC\u2010VICs co\u2010cultured with ECFCs expressed significantly higher levels of the perivascular markers \u03b1SMA and calponin compared to C\u2010VICs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001 and\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05, respectively). In conclusion, our study reveals the heterogeneity in VIC plasticity within the aortic valve during CAVS. The diminished capacity of VICs from calcified areas to differentiate into perivascular cells suggests a loss of function as valve disease progresses. Furthermore, the ability of VICs to undergo perivascular differentiation may provide insights into valve homeostasis, angiogenesis and the exacerbation of calcification.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70511","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T21:44:52Z","timestamp":1743457492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unveiling the Angiogenic Potential and Functional Decline of Valve Interstitial Cells During Calcific Aortic Valve Stenosis Progression"],"prefix":"10.1111","volume":"29","author":[{"given":"Adeline","family":"Blandini\u00e8res","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"},{"name":"AP\u2010HP, European Georges Pompidou Hospital Hematology Department  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Rossi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Gendron","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"},{"name":"AP\u2010HP, European Georges Pompidou Hospital Hematology Department  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne","family":"Rancic","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mickael","family":"Rosa","sequence":"additional","affiliation":[{"name":"Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011\u2010EGID  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annabelle","family":"Dupont","sequence":"additional","affiliation":[{"name":"Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011\u2010EGID  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salim","family":"Idelcadi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aur\u00e9lien","family":"Philippe","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"},{"name":"AP\u2010HP, European Georges Pompidou Hospital Hematology Department  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bastien","family":"Poitier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"},{"name":"AP\u2010HP, European Georges Pompidou Hospital Cardiac Surgery Department  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Bi\u00e8che","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Pharmacogenomics Unit, Department of Genetics  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Vacher","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Pharmacogenomics Unit, Department of Genetics  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Cholley","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"},{"name":"AP\u2010HP, European Georges Pompidou Hospital Department of Anesthesia and Intensive Care  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale","family":"Gaussem","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 Innovative Therapies in Haemostasis, INSERM  Paris France"},{"name":"AP\u2010HP, European Georges Pompidou Hospital Hematology Department  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Susen","sequence":"additional","affiliation":[{"name":"Univ. 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