{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:09:21Z","timestamp":1791310161234,"version":"4.3.1"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071629"],"award-info":[{"award-number":["82071629"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to elucidate the roles of junctional adhesion molecule 2 (JAM\u20102), collagen I and matrix metalloproteinase 2 (MMP\u20102) in the pathogenesis of pelvic organ prolapse (POP) and explore their potential as diagnostic markers. We examined 82 POP patients and 64 controls using enzyme\u2010linked immunosorbent assay (ELISA) and quantitative Polymerase Chain Reaction (qPCR) to analyse protein and gene expression levels of JAM\u20102, Collagen I and MMP\u20102. Receiver operating characteristic (ROC) analysis evaluated their diagnostic efficacy, with correlation analyses linking molecular markers to POP severity based on POP\u2010Q grades. Our study found no significant differences in age, BMI and vaginal parity between POP patients and controls. Molecular analyses revealed significant alterations in the expression levels of JAM\u20102, Collagen I and MMP\u20102 in POP patients. Specifically, there was a marked decrease in JAM\u20102 and collagen I levels, accompanied by an increase in MMP\u20102 expression, indicating a disruption in the balance between tissue synthesis and degradation. ROC analysis demonstrated the significant discriminative power of these markers, with substantial area under the curve (AUC) values for diagnosing POP. Correlation analysis further showed a significant association between the expression of JAM\u20102, Collagen I and MMP\u20102 and the clinical severity of POP, as indicated by POP\u2010Q grades. Our findings revealed the significant changes in the expression of JAM\u20102, Collagen I and MMP\u20102 that may contribute to the POP pathogenesis. The diagnostic potential of these markers was substantiated, suggesting their utility in developing noninvasive diagnostic tools for POP.<\/jats:p>","DOI":"10.1111\/jcmm.70512","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T21:38:20Z","timestamp":1743457100000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Clinical Significance of\n                    <scp>JAM<\/scp>\n                    \u20102 Expression in the Vaginal Wall Tissues of Patients With Pelvic Organ Prolapse"],"prefix":"10.1111","volume":"29","author":[{"given":"Yuan","family":"Liu","sequence":"first","affiliation":[{"name":"The International Peace Maternity &amp; Child Health Hospital of China Welfare Institute (IPMCH) Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajing","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"Clinical Research Unit, Shanghai First Maternity and Infant Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"Jiading Maternal and Child Health Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"Clinical Research Unit, Shanghai First Maternity and Infant Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Wan","sequence":"additional","affiliation":[{"name":"The International Peace Maternity &amp; Child Health Hospital of China Welfare Institute (IPMCH) Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyi","family":"Gu","sequence":"additional","affiliation":[{"name":"The International Peace Maternity &amp; Child Health Hospital of China Welfare Institute (IPMCH) Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Peng","sequence":"additional","affiliation":[{"name":"The International Peace Maternity &amp; Child Health Hospital of China Welfare Institute (IPMCH) Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Hainan Branch, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Jiao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"Hainan Branch, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3344-5236","authenticated-orcid":false,"given":"Xiaolin","family":"Hua","sequence":"additional","affiliation":[{"name":"The International Peace Maternity &amp; Child Health Hospital of China Welfare Institute (IPMCH) Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Hainan Branch, Shanghai Children's Medical Center, School of Medicine Shanghai Jiao Tong University  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,30]]},"reference":[{"key":"e_1_2_12_1_2_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10442"},{"key":"e_1_2_12_1_3_1","volume-title":"Pelvic Organ Prolapse","author":"Aboseif C.","year":"2024"},{"key":"e_1_2_12_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20012"},{"key":"e_1_2_12_1_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12010094"},{"key":"e_1_2_12_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.02.033"},{"key":"e_1_2_12_1_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-1591.32064"},{"key":"e_1_2_12_1_8_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11564"},{"key":"e_1_2_12_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u201094\u2010007\u20106211\u20104_2"},{"key":"e_1_2_12_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2019.01.012"},{"key":"e_1_2_12_1_11_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050399"},{"key":"e_1_2_12_1_12_1","doi-asserted-by":"publisher","DOI":"10.11604\/pamj.2018.30.283.9905"},{"key":"e_1_2_12_1_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2000.7642"},{"key":"e_1_2_12_1_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2016.3340"},{"key":"e_1_2_12_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2009.03100.x"},{"key":"e_1_2_12_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-005-0045-y"},{"key":"e_1_2_12_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.150"},{"key":"e_1_2_12_1_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/br.2016.736"},{"key":"e_1_2_12_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2019.02.007"},{"key":"e_1_2_12_1_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000001448"},{"key":"e_1_2_12_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-021-04834-7"},{"key":"e_1_2_12_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-009-0895-9"},{"key":"e_1_2_12_1_23_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.4773"},{"key":"e_1_2_12_1_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020449"},{"key":"e_1_2_12_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.08.059"},{"key":"e_1_2_12_1_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajo.12169"},{"key":"e_1_2_12_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1400"},{"key":"e_1_2_12_1_28_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2467"},{"key":"e_1_2_12_2_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-022-03587-z"},{"key":"e_1_2_12_2_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2021.1945574"},{"key":"e_1_2_12_2_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-022-04415-6"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.70512","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:22:48Z","timestamp":1772983368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,30]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/jcmm.70512"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70512","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,30]]},"assertion":[{"value":"2024-07-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70512"}}