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Autophagy is known to modulate the expression of intestinal solute transporters; however, its role in regulating PAT\u20101 has not been examined. Autophagy in Caco\u20102 cells was induced by either rapamycin treatment or by nutrient deprivation and assessed by conventional autophagy marker proteins. ATG7 (autophagy\u2010related 7) protein expression was attenuated by ATG7\u2010siRNA in Caco\u20102 cells or by utilising ATG7KO mice. PAT\u20101 protein levels in Caco\u20102 cells were significantly reduced by rapamycin or by nutrient deprivation at 48 and 72\u2009h. Concomitantly, the LC3II\/I ratio was increased, and p62 levels were significantly decreased, confirming the induction of autophagy. Nutrient deprivation for 6\u2009h also caused a significant decrease in the surface levels of PAT\u20101. PAT\u20101 protein levels were increased by the siRNA\u2010mediated ATG7 knockdown in Caco\u20102 cells and in the ileum of ATG7KO mice. In summary, Autophagy in intestinal epithelial cells modulates the basal levels of PAT\u20101 protein and may play a critical role in the maintenance of oxalate homeostasis.<\/jats:p>","DOI":"10.1111\/jcmm.70513","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T10:20:13Z","timestamp":1746181213000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Autophagy Regulates Putative Anion Transporter 1 Expression in Intestinal Epithelial Cells"],"prefix":"10.1111","volume":"29","author":[{"given":"Shubha","family":"Priyamvada","sequence":"first","affiliation":[{"name":"Division of Gastroenterology &amp; Hepatology, Department of Medicine University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dulari","family":"Jayawardena","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology &amp; Hepatology, Department 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