{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T23:47:43Z","timestamp":1776296863406,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["110\u20102628\u2010B\u2010371\u2010001"],"award-info":[{"award-number":["110\u20102628\u2010B\u2010371\u2010001"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["111\u20102628\u2010B\u2010371\u2010001"],"award-info":[{"award-number":["111\u20102628\u2010B\u2010371\u2010001"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["112\u20102628\u2010B\u2010371\u2010001"],"award-info":[{"award-number":["112\u20102628\u2010B\u2010371\u2010001"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007632","name":"Changhua Christian Hospital","doi-asserted-by":"publisher","award":["112\u2010CCH\u2010IRP\u2010014"],"award-info":[{"award-number":["112\u2010CCH\u2010IRP\u2010014"]}],"id":[{"id":"10.13039\/501100007632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Long\u2010term peritoneal dialysis (PD) leads to peritoneal damage and chronic inflammation, resulting in peritoneal fibrosis (PF). Emerging evidence suggests that yes\u2010associated protein (YAP) is a key player in fibrogenesis across various organs. However, its role in PD\u2010induced PF remains unclear. We used NIH\/3T3 cells, primary mouse fibroblasts, and conditional YAP knockout (CKO) mice with glioma\u2010associated oncogene 1 (<jats:italic>Gli1<\/jats:italic>)\u2010specific YAP deletion. The effects of YAP knockdown and verteporfin, a YAP inhibitor, on fibroblast\u2010to\u2010mesenchymal transition (FMT) and angiogenesis were evaluated. Transforming growth factor\u2010beta (TGF\u2010\u03b2) induced YAP expression and promoted fibroblast\u2010to\u2010myofibroblast transition (FMT) in 3T3 fibroblasts, upregulating collagen 1A1, \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), and connective tissue growth factor (CTGF). YAP knockdown and verteporfin treatment reduced these FMT markers and inhibited smad2\/3 phosphorylation. In\u00a0vivo, YAP and <jats:italic>Gli1<\/jats:italic>\u2010expressing cells were upregulated in PD\u2010induced PF. Conditional YAP knockout in <jats:italic>Gli1<\/jats:italic><jats:sup>+<\/jats:sup> cells and verteporfin treatment significantly reduced fibrosis and \u03b1\u2010SMA, collagen 1, TGF\u2010\u03b2, CTGF, and phosphorylated smad2\/3 expression in the peritoneum and peritoneal angiogenesis. YAP plays a pivotal role in FMT during PD\u2010induced PF. Conditional YAP deletion in <jats:italic>Gli1<\/jats:italic>\u2010expressing cells and verteporfin treatment represent promising antifibrotic strategies for long\u2010term PD patients.<\/jats:p>","DOI":"10.1111\/jcmm.70516","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T05:30:25Z","timestamp":1743139825000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Depleting Yes\u2010Associated Protein in <i>Gli1<\/i>\u2010Expressing Cells Attenuates Peritoneal Dialysis\u2010Induced Peritoneal Fibrosis"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6045-6956","authenticated-orcid":false,"given":"Chia\u2010Lin","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Post\u2010Baccalaureate Medicine, College of Medicine National Chung Hsing University  Taichung Taiwan"},{"name":"School of Medicine Chung Shan Medical University  Taichung Taiwan"},{"name":"Division of Nephrology, Department of Internal Medicine Changhua Christian Hospital  Changhua Taiwan"},{"name":"Renal Medicine Laboratory Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jhih\u2010Wen","family":"Hsu","sequence":"additional","affiliation":[{"name":"Renal Medicine Laboratory Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Chi","family":"Chan","sequence":"additional","affiliation":[{"name":"Renal Medicine Laboratory Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenn\u2010Yah","family":"Yu","sequence":"additional","affiliation":[{"name":"National Yang Ming Chiao Tung University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Liang","family":"Tsai","sequence":"additional","affiliation":[{"name":"Renal Medicine Laboratory Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Der\u2010Cherng","family":"Tarng","sequence":"additional","affiliation":[{"name":"Department and Institute of Physiology National Yang Ming Chiao Tung University  Taipei Taiwan"},{"name":"Division of Nephrology, Department of Medicine Taipei Veterans General Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,25]]},"reference":[{"issue":"2","key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"S59","DOI":"10.1177\/089686080902902S11","article-title":"Clinical Advantages of Peritoneal Dialysis","volume":"29","author":"Tokgoz B.","year":"2009","journal-title":"Peritoneal Dialysis International"},{"issue":"6","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1053\/j.ackd.2011.09.001","article-title":"Peritoneal Dialysis Versus Hemodialysis: Risks, Benefits, and Access Issues","volume":"18","author":"Sinnakirouchenan R.","year":"2011","journal-title":"Advances in Chronic Kidney Disease"},{"issue":"15","key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"7955","DOI":"10.3390\/ijms22157955","article-title":"How to Improve the Biocompatibility of Peritoneal Dialysis Solutions (Without Jeopardizing the Patient's Health)","volume":"22","author":"Bonomini M.","year":"2021","journal-title":"International Journal of Molecular Sciences"},{"issue":"1","key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1159\/000368972","article-title":"Ultrafiltration Failure in Peritoneal Dialysis: A Pathophysiologic Approach","volume":"39","author":"Teitelbaum I.","year":"2015","journal-title":"Blood Purification"},{"issue":"1","key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1177\/08968608221125606","article-title":"Encapsulating Peritoneal Sclerosis: Your Questions Answered","volume":"43","author":"Pepereke S.","year":"2023","journal-title":"Peritoneal Dialysis International"},{"issue":"2","key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1158\/1541-7786.MCR-15-0305","article-title":"Control of Proliferation and Cancer Growth by the Hippo Signaling Pathway","volume":"14","author":"Ehmer U.","year":"2016","journal-title":"Molecular Cancer Research"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1909210"},{"issue":"4","key":"e_1_2_11_9_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/s41580-018-0086-y","article-title":"Hippo\u2010YAP\/TAZ Signalling in Organ Regeneration and Regenerative Medicine","volume":"20","author":"Moya I. 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Renal Physiology"},{"issue":"1","key":"e_1_2_11_31_1","doi-asserted-by":"crossref","first-page":"5392","DOI":"10.1038\/s41598-017-05624-2","article-title":"Inhibition of CTGF Ameliorates Peritoneal Fibrosis Through Suppression of Fibroblast and Myofibroblast Accumulation and Angiogenesis","volume":"7","author":"Sakai N.","year":"2017","journal-title":"Scientific Reports"},{"issue":"1","key":"e_1_2_11_32_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1128\/MCB.00772-15","article-title":"Myocardin\u2010Related Transcription Factor A and Yes\u2010Associated Protein Exert Dual Control in G Protein\u2010Coupled Receptor\u2010 and RhoA\u2010Mediated Transcriptional Regulation and Cell Proliferation","volume":"36","author":"Yu O. M.","year":"2015","journal-title":"Molecular and Cellular Biology"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S302466"},{"issue":"3","key":"e_1_2_11_34_1","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1038\/s41401-020-0463-x","article-title":"KLF4 Initiates Sustained YAP Activation to Promote Renal Fibrosis in Mice After Ischemia\u2010Reperfusion Kidney Injury","volume":"42","author":"Xu D.","year":"2021","journal-title":"Acta Pharmacologica Sinica"},{"key":"e_1_2_11_35_1","doi-asserted-by":"crossref","first-page":"6943","DOI":"10.1038\/ncomms7943","article-title":"Yes\u2010Associated Protein Regulates Endothelial Cell Contact\u2010Mediated Expression of Angiopoietin\u20102","volume":"6","author":"Choi H. J.","year":"2015","journal-title":"Nature Communications"},{"issue":"2","key":"e_1_2_11_36_1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s12079-017-0390-x","article-title":"Tamoxifen for Induction of Cre\u2010Recombination May Confound Fibrosis Studies in Female Mice","volume":"11","author":"Falke L. L.","year":"2017","journal-title":"Journal of Cell Communication and Signaling"},{"issue":"1","key":"e_1_2_11_37_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s10020-019-0110-5","article-title":"Tamoxifen and Bone Morphogenic Protein\u20107 Modulate Fibrosis and Inflammation in the Peritoneal Fibrosis Model Developed in Uremic Rats","volume":"25","author":"Silva F. M. O.","year":"2019","journal-title":"Molecular Medicine"},{"issue":"9","key":"e_1_2_11_38_1","first-page":"2350","article-title":"Comparative Evaluation of Inducible Cre Mouse Models for Fibroblast Targeting in the Healthy and Infarcted Myocardium","volume":"10","author":"Aguado\u2010Alvaro L. 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