{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T12:23:48Z","timestamp":1779193428267,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["242300420088"],"award-info":[{"award-number":["242300420088"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372895"],"award-info":[{"award-number":["82372895"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pancreatic cancer is one of the deadliest cancers. Chemokines affect the progression of pancreatic cancer through various mechanisms. Most of the CXC chemokine genes, CC chemokine genes and CX3C chemokine genes are clustered together within a very short region of chromatin. Transcription activity of gene clusters is usually influenced by the chromatin architecture and spatial organisation. Nevertheless, the chromatin\u2010mediated regulatory mechanism on transcription of chemokine gene clusters has never been studied in pancreatic cancer. Herein, we determined that the expression of C\u2010X\u2010C motif chemokine ligand 8 (\n                    <jats:italic>CXCL8<\/jats:italic>\n                    ),\n                    <jats:italic>CXCL6<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL4L1<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL1<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL4<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL7<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL5<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL3<\/jats:italic>\n                    and\n                    <jats:italic>CXCL2<\/jats:italic>\n                    was up\u2010regulated, whereas\n                    <jats:italic>CXCL9<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL10<\/jats:italic>\n                    and\n                    <jats:italic>CXCL11<\/jats:italic>\n                    were down\u2010regulated in pancreatic cancer cells compared with normal duct epithelial cells and further uncovered that four enhancer elements showed robust interaction to form DNA looping containing the up\u2010regulated eight\n                    <jats:italic>CXCL<\/jats:italic>\n                    genes, whereas the other enhancer controlled\n                    <jats:italic>CXCL9<\/jats:italic>\n                    ,\n                    <jats:italic>CXCL10<\/jats:italic>\n                    and\n                    <jats:italic>CXCL11<\/jats:italic>\n                    to form another DNA loop. Furthermore, after these enhancers were respectively destroyed by CRISPR\u2010Cas9, we observed that the interaction with other enhancers was weakened as well as the expression of\n                    <jats:italic>CXCL<\/jats:italic>\n                    gene clusters and the tumour malignancy of pancreatic cancer cells was significantly changed. Taken together, our research exhibits the regulatory mechanism on transcription of\n                    <jats:italic>CXCL<\/jats:italic>\n                    gene clusters via enhance\u2010dependent DNA looping alteration in pancreatic cancer cells.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70538","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T23:43:38Z","timestamp":1744069418000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>CXCL<\/scp>\n                    Gene Clusters Regulated by Enhancer\u2010Mediated\n                    <scp>DNA<\/scp>\n                    Looping Alteration in Pancreatic Cancer Cells"],"prefix":"10.1111","volume":"29","author":[{"given":"Yifen","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Xi'an Jiaotong University, Pancreas Center of Xi'an Jiaotong University, Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Central Laboratory Suzhou Ninth People's Hospital  Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanping","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology Suzhou Vocational Health College  Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genhai","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of General Surgery Suzhou Ninth People's Hospital  Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2923-1641","authenticated-orcid":false,"given":"Yihang","family":"Shen","sequence":"additional","affiliation":[{"name":"Central Laboratory Suzhou Ninth People's Hospital  Suzhou Jiangsu China"},{"name":"Department of General Surgery Suzhou Ninth People's Hospital  Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0490-466X","authenticated-orcid":false,"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Xi'an Jiaotong University, Pancreas Center of Xi'an Jiaotong University, Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30974-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2019.1607294"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2016.11.57"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2022.08.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1154581"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2017.05.012"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2024.03.009"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.108598"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2019-000308"},{"key":"e_1_2_9_11_1","first-page":"5197","article-title":"Protocadherin\u20101 Serves as a Prognostic Biomarker and Promotes Pancreatic Cancer Progression by Suppressing CD8(+) T Cell Infiltration Through CCL5\u2010CCR5 Axis","volume":"13","author":"Jiang Z.","year":"2023","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2021.07.008"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/19491034.2022.2143106"},{"key":"e_1_2_9_14_1","unstructured":"T.Needs L. F.Gonzalez\u2010Mosquera andD. T.Lynch \u201cBeta Thalassemia. StatPearls. Treasure Island (FL) Ineligible Companies. Disclosure: Luis Gonzalez\u2010Mosquera Declares No Relevant Financial Relationships With Ineligible Companies. Disclosure: David Lynch Declares No Relevant Financial Relationships With Ineligible Companies.\u201d(2024)."},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa197"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa142"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13039\u2010018\u20100368\u20102"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1114"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-023-01450-7"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23020792"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2024.176357"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12010"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2016.08.002"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2017.11.007"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201009\u20102329"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2018.02.022"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.278565.123"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-023-01032-x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20200014"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/21541264.2023.2222032"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021368\u20100"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70538","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.70538","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70538","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:22:45Z","timestamp":1772983365000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/jcmm.70538"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70538","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"2025-01-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70538"}}