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We used conventional molecular biology technology and multi\u2010photon confocal live imaging to explore the effect of the interactive dialogue between iNKT and Kupffer cells on neutrophil recruitment in the occurrence and development of APAP\u2010induced liver injury. iNKT cell deficient mice were more prone to liver injury induced by excessive APAP, and the degree of liver injury was more severe. After injury, iNKT cells were recruited into the liver and showed IL\u20104 high expression activation mode. Furthermore, we also found that Kupffer cells in APAP induced liver injury produce M1 polarisation and highly expressed IL\u201012, that Kupffer cells regulate the activation of iNKT cells through IL\u201012; and that IL\u20104 produced by iNKT cells in the liver can also inhibit inflammatory damage caused by neutrophil recruitment. Our study shows that Kupffer cells produce IL\u201012 and promote activated iNKT cells to produce more IL\u20104, and inhibit the recruitment of neutrophils, thereby reducing the degree of liver inflammatory injury induced by APAP.<\/jats:p>","DOI":"10.1111\/jcmm.70549","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T09:58:05Z","timestamp":1751450285000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Kupffer Cells Regulate <scp>iNKT<\/scp> Cells Through Il\u201012 to Mitigate the Extent of <scp>APAP<\/scp>\u2010Induced Damage in the Liver"],"prefix":"10.1111","volume":"29","author":[{"given":"Yinling","family":"Li","sequence":"first","affiliation":[{"name":"Department of School of Life Sciences Zhejiang University of Traditional Chinese Medicine  Zhejiang Hangzhou China"},{"name":"Department of Translational Medicine Platform The Affiliated Hospital of Hangzhou Normal 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