{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T20:18:59Z","timestamp":1767730739819,"version":"build-2238731810"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Considering the different behaviour of cells in response to diseases in different conditions and sex hormone\u2010dependent cancers, we addressed the possible effect of the sex of the source of these cells from adipose tissue on prostate cancer cells. In this in\u00a0vitro study, we evaluated the effects of male and female MSC Conditioned Media (MCM, FCM) on prostate cancer cells. The assessment included Hoechst dye staining, a scratch\u2010wound assay, a colony formation assay, and a flow cytometric analysis of apoptosis and the cell cycle. We also performed real\u2010time PCR to examine various genes, including apoptosis\u2010related genes, epithelial\u2010mesenchymal transition (\n                    <jats:italic>EMT<\/jats:italic>\n                    ) genes, angiogenesis\u2010related genes, and cell growth and survival biomarkers. Our results indicated that the IC\n                    <jats:sub>50<\/jats:sub>\n                    values were 50% and 75% media in MCM and FCM in each of the three prostate cancer cell lines, respectively. An evaluation of gene expression revealed that in all three prostate cancer cell lines, treatment with MCM was more effective than FCM in reducing the expression of\n                    <jats:italic>N\u2010Cadherin<\/jats:italic>\n                    and\n                    <jats:italic>Vimentin<\/jats:italic>\n                    ,\n                    <jats:italic>EGFR<\/jats:italic>\n                    and\n                    <jats:italic>BCL2<\/jats:italic>\n                    genes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Furthermore, the MCM significantly increased the expression of\n                    <jats:italic>BAX<\/jats:italic>\n                    and\n                    <jats:italic>E\u2010Cadherin<\/jats:italic>\n                    genes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) in the PC3 cell line. MCM proved to be more effective than FCM in reducing the expression of the epithelial\u2010mesenchymal transition pathway,\n                    <jats:italic>EGFR<\/jats:italic>\n                    gene, and Apoptosis Regulator (\n                    <jats:italic>BCL2<\/jats:italic>\n                    ) in the PC3 cell line. Due to its potential in regenerative medicine and cell therapy, this approach may serve as an effective treatment option for advanced prostate cancer.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70569","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T23:53:42Z","timestamp":1747094022000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["RETRACTED: Sex\u2010Dependent Paracrine Effect of Conditioned Media From Adipose Tissue Derived Mesenchymal Stem Cells on Prostate Cancer Cells"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2219-999X","authenticated-orcid":false,"given":"Akram","family":"Mirzaei","sequence":"first","affiliation":[{"name":"Urology Research Center Tehran University of Medical Sciences  Tehran 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