{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T14:19:03Z","timestamp":1762525143348,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","award":["18JCYBJC27200"],"award-info":[{"award-number":["18JCYBJC27200"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82400166\uff1a82270142","82000128","81970115","81770110"],"award-info":[{"award-number":["82400166\uff1a82270142","82000128","81970115","81770110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019065","name":"Tianjin Municipal Science and Technology Program","doi-asserted-by":"publisher","award":["24ZXGZSY00090"],"award-info":[{"award-number":["24ZXGZSY00090"]}],"id":[{"id":"10.13039\/501100019065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Secondary gene mutations are one of the main mechanisms underlying paroxysmal nocturnal haemoglobinuria (PNH) clone proliferation. Our previous studies showed that SUZ12 participates in PNH clone proliferation by regulating H3K27me3. However, the mechanism underlying the SUZ12 function remains unclear. Immunoprecipitation and mass spectrometry were used to identify SUZ12 interacting proteins in\n                    <jats:italic>PIGA<\/jats:italic>\n                    \u2010KO K562 cells. Furthermore, RNA\u2010seq was used to explore the signalling pathways. Finally, colony formation assays, western blotting, and flow cytometry were performed to determine the proliferative ability of the cells. We identified 59 potential proteins that interact with SUZ12 and revealed a physical interaction between MIB2 and SUZ12 exclusively in the K562\u2010KO cell line. Furthermore, we emphasise the vital involvement of the MIB\/HERC and ZZ\u2010type domains in the physical association between MIB2 and SUZ12. After MIB2 knockdown, SUZ12 protein decreased while the ubiquitination levels of SUZ12 were enhanced. Additionally, PRC2\u2010related target genes were upregulated in the siMIB2 group. SUZ12 and H3K27me3 expression levels and cell proliferation significantly decreased after MIB2 knockdown, whereas cell apoptosis significantly increased. MIB2 protein levels are also elevated in patients with PNH. In conclusion, MIB2 affects the stability of the PRC2 complex by mediating SUZ12 ubiquitination, which in turn regulates PNH clone proliferation.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70597","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T11:25:50Z","timestamp":1749727550000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>MIB2<\/scp>\n                    \u2010Mediated\n                    <scp>SUZ12<\/scp>\n                    Ubiquitination Regulates Clonal Proliferation in Patients With Paroxysmal Nocturnal Haemoglobinuria"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1969-6015","authenticated-orcid":false,"given":"Hui","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin China"},{"name":"Tianjin Institute of Hematology  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijie","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin China"},{"name":"Tianjin Institute of Hematology  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaomeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin China"},{"name":"Tianjin Institute of Hematology  Tianjin 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