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Hypoxia\u2010inducible factor 1\u2010alpha (HIF1\u03b1) is a subunit of hypoxia\u2010inducible factor 1 (HIF1), which plays a critical regulatory role in cellular responses under hypoxic conditions. Moreover, the degradation pathway of HIF1\u03b1 is regulated by the deubiquitinase Ovarian Tumour Domain\u2010containing Ubiquitin Aldehyde Binding Protein 1 (OTUB1). The aim of this study is to investigate the therapeutic effects of EPH on ALI and its potential therapeutic mechanism. We utilised a lipopolysaccharide (LPS)\u2010induced ALI mouse model and employed various methods for evaluation. Ultimately, our research findings demonstrate that EPH exhibits anti\u2010ALI effects, with the involvement of HIF1\u03b1 and OTUB1 in the pharmacological actions of EPH. In conclusion, our study results demonstrate that EPH exhibits anti\u2010ALI effects and exerts its protective effects through modulation of the OTUB1 and HIF1\u03b1 pathways. Our research findings not only lay the foundation for expanding the medicinal applications of EPH but also provide direction for seeking improved treatment strategies for ALI.<\/jats:p>","DOI":"10.1111\/jcmm.70598","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T13:41:37Z","timestamp":1747662097000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ephedrine Attenuates <scp>LPS<\/scp>\u2010Induced Acute Lung Injury in Mice by Inhibiting <scp>OTUB1<\/scp> and Promoting <scp>K48<\/scp> Ubiquitination of <scp>HIF1\u03b1<\/scp>"],"prefix":"10.1111","volume":"29","author":[{"given":"Bo","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Pharmacy, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education Ningxia Medical University  Yinchuan Ningxia 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