{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:35:58Z","timestamp":1791909358322,"version":"4.3.4"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Waldenstr\u00f6m Macroglobulinaemia (WM) is sometimes difficult to differentiate from marginal zone lymphoma (MZL), two entities with overlapping features for which no single marker assessed by multiparameter flow cytometry (MFC) is specific. The aim of this work was to establish a diagnostic phenotypic score for bone marrow (BM) and peripheral blood (PB) WM samples, to differentiate it from MZL and to improve the detection of small circulating WM clones. This study revealed a distinct phenotypic profile between WM and MZL B\u2010cells. WM B\u2010cells showed decreased expression of CD19, FMC7, CD22, CD27 and increased expression of CD79b and CD13. Supervised and unsupervised MFC analyses were used to define a phenotypic scoring system: a score of 3\/6 or greater in BM or 4\/7 or greater in PB samples supported the diagnosis of WM (sensitivity of 97.9% and 94.1% and specificity of 80.0% and 93.5%, respectively). These results were validated in a prospective cohort with very high sensitivity and specificity for the two scoring systems. Clinico\u2010biological correlations showed that the absence or low expression of CD38 on BM WM B\u2010cells was significantly associated with increased BM and PB infiltration (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001 and <jats:italic>p<\/jats:italic>\u2009=\u20090.0024 respectively) and <jats:italic>CXCR4<\/jats:italic> mutation (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). These results demonstrate that MFC can be used to differentiate WM from MZL with a scoring system that can be easily implemented in routine practice.<\/jats:p>","DOI":"10.1111\/jcmm.70620","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T13:49:52Z","timestamp":1748008192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Phenotypic Profile of Waldenstr\u00f6m Macroglobulinaemia B\u2010Cells: Establishment of a Diagnosis Scoring System and Clinico\u2010Biological Correlations"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3644-1353","authenticated-orcid":false,"given":"Elise","family":"Sourdeau","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, Service d'H\u00e9matologie Biologique, H\u00f4pital Piti\u00e9\u2010Salp\u00eatri\u00e8re APHP  Paris France"},{"name":"Centre de Recherche des Cordeliers Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9, Inserm UMRS 1138, Drug Resistance in Hematological Malignancies Team  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mentine","family":"Boccon\u2010Gibod","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Service d'H\u00e9matologie Clinique, H\u00f4pital Piti\u00e9\u2010Salp\u00eatri\u00e8re APHP  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1272-6872","authenticated-orcid":false,"given":"Aur\u00e9lien","family":"Corneau","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM UMS037 PASS, Plateforme de Cytom\u00e9trie (CyPS)  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myrto","family":"Costopoulos","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Service d'H\u00e9matologie Biologique, H\u00f4pital 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