{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T15:00:11Z","timestamp":1763564411236,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007632","name":"Changhua Christian Hospital","doi-asserted-by":"publisher","award":["112\u2010CCH\u2010IRP\u2010006","113\u2010CCH\u2010IRP\u2010004"],"award-info":[{"award-number":["112\u2010CCH\u2010IRP\u2010006","113\u2010CCH\u2010IRP\u2010004"]}],"id":[{"id":"10.13039\/501100007632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Nasopharyngeal carcinoma (NPC) is a unique cancer type originating from the nasopharynx. To investigate novel strategies for improving prognosis and reducing the adverse effects of current treatments, this study examined the efficacy of hellebrigenin. Hellebrigenin demonstrated selective cytotoxicity against NPC\u2010BM and NPC\u2010039 cell lines without harming normal nasopharyngeal cells. Treatment with hellebrigenin resulted in G2\/M cell cycle arrest in both NPC cell lines. The apoptotic phenomena induced by hellebrigenin included chromatin condensation, increased apoptotic cells and altered mitochondrial membrane potential. Proteomics analysis and the bioinformatic data identified coiled\u2010coil\u2010helix\u2010coiled\u2010coil\u2010helix domain containing 2 (CHCHD2) as a candidate oncogene in NPC. Moreover, the combination of CHCHD2 siRNA, CHCHD2 plasmid and hellebrigenin pointed out that CHCHD2 could be a critical mediator of hellebrigenin\u2010induced apoptosis. The combined treatment of hellebrigenin with mitogen\u2010activated protein kinase inhibitors revealed the involvement of the extracellular signal\u2013regulated kinases and c\u2010Jun N\u2010terminal kinases pathways in hellebrigenin\u2010induced apoptosis in NPC cells. In\u00a0vivo studies demonstrated that hellebrigenin suppressed the tumour volume without affecting body weight, accompanied by the downregulation of Ki67 and CHCHD2 expression. In conclusion, this study provides evidence that hellebrigenin induces NPC apoptosis through regulating CHCHD2 both in\u00a0vitro and in\u00a0vivo.<\/jats:p>","DOI":"10.1111\/jcmm.70624","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T09:49:16Z","timestamp":1748425756000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Efficacy of Hellebrigenin Against Nasopharyngeal Carcinoma Cells: The Molecular and Bioinformatic Analysis"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8496-1313","authenticated-orcid":false,"given":"Hsin\u2010Yu","family":"Ho","sequence":"first","affiliation":[{"name":"Oral Cancer Research Center Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mu\u2010Kuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery Changhua Christian Hospital  Changhua Taiwan"},{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Chung Hsing University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Jung","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Changhua Christian Hospital  Changhua Taiwan"},{"name":"Translational Pathology Core Laboratory Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2746-4054","authenticated-orcid":false,"given":"Chia\u2010Chieh","family":"Lin","sequence":"additional","affiliation":[{"name":"Oral Cancer Research Center Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Sheng","family":"Lo","sequence":"additional","affiliation":[{"name":"Oral Cancer Research Center Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Ching","family":"Chuang","sequence":"additional","affiliation":[{"name":"Oral Cancer Research Center Changhua Christian Hospital  Changhua Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9726-3234","authenticated-orcid":false,"given":"Ming\u2010Ju","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Oral Cancer Research Center Changhua Christian Hospital  Changhua Taiwan"},{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Chung Hsing University  Taichung Taiwan"},{"name":"Graduate Institute of Biomedical Sciences China Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055\u20109965.EPI\u201006\u20100353"},{"key":"e_1_2_11_3_1","first-page":"721","volume-title":"Cancer Registry Annual Report, 2021, Taiwan","author":"Health Promotion Administration","year":"2023"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2008.09.008"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S341472"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578x231201053"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2012.1683"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010017\u20109525\u20101"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2011.09.010"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2018.4567"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.711220"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.9011"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0165\u20104608(97)00416\u20100"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165\u20104608(90)90161\u20103"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010020\u20100546\u20108"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20210854"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddaa077"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/23723556.2015.1004964"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072682"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.18071"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2014.06.003"},{"key":"e_1_2_11_22_1","first-page":"566","article-title":"An Indolocarbazole Inhibitor of Human Checkpoint Kinase (Chk1) Abrogates Cell Cycle Arrest Caused by DNA Damage","volume":"60","author":"Jackson J. 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