{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T11:46:52Z","timestamp":1784029612049,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["ASTIP"],"award-info":[{"award-number":["ASTIP"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["CAAS\u2010SCAB\u2010202301"],"award-info":[{"award-number":["CAAS\u2010SCAB\u2010202301"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The ubiquitin ligase RING finger 20 (RNF20) mediated the monoubiquitination of histone H2B at lysine 120 (H2Bub), an epigenetic modification known to regulate key biological processes such as fat tissue development, tumorigenesis, spermatogenesis and so on. Despite our previous findings showing that mice with adipocyte\u2010specific deletion of\n                    <jats:italic>Rnf20<\/jats:italic>\n                    (ASKO mice) develop hyperinsulinaemia, the underlying mechanisms remain unclear. In this study, we investigated the role of adipocyte RNF20 in maintaining systemic insulin homoeostasis in ASKO mice. Our results reveal that ASKO mice exhibit an enlarged pancreas, increased islet size and a greater number of pancreatic \u03b2\u2010cells. Fat tissue in ASKO mice showed reduced insulin sensitivity, evidenced by diminished AKT phosphorylation under basal and insulin\u2010stimulated conditions, alongside suppressed insulin signalling pathways. Furthermore, the decreased levels of histone modifications, including H2Bub, H3K4me3 and H3K79me3, were observed in both ASKO mice fat tissues and\n                    <jats:italic>Rnf20<\/jats:italic>\n                    \u2010knockdown 3T3\u2010L1 cells. Mechanistically,\n                    <jats:italic>Rnf20<\/jats:italic>\n                    knockdown in adipocytes reduced H3K4me3 occupancy at the\n                    <jats:italic>Slc2a4<\/jats:italic>\n                    gene locus, inhibiting GLUT4 expression and inducing adipose\u2010specific insulin resistance. These findings establish a critical role for adipocyte RNF20 in the insulin signalling regulation via the H2Bub\u2010H3K4me3\u2010\n                    <jats:italic>Slc2a4<\/jats:italic>\n                    axis, highlighting its importance in systemic glucose metabolism.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70649","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T07:34:32Z","timestamp":1750145672000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Adipocyte\n                    <scp>RNF20<\/scp>\n                    Knockout Leads to Hyperinsulinemia via the\n                    <scp>H2Bub<\/scp>\n                    \u2010\n                    <scp>H3K4me3<\/scp>\n                    \u2010\n                    <i>Slc2a4<\/i>\n                    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Medical School University of Chinese Academy of Sciences  Beijing China"},{"name":"Sanya Institute, Hainan Academy of Agricultural Sciences  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxiang","family":"Yuan","sequence":"additional","affiliation":[{"name":"Lin He's Academician Workstation of New Medicine and Clinical Translation Jining Medical University  Jining Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Medical Imaging and Laboratory Jining Medical University  Jining Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1492-6771","authenticated-orcid":false,"given":"Yanfang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding Institute of Animal Science, Chinese Academy of Agricultural Sciences  Beijing 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