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Identification of a druggable internalising cell\u2010surface target enables the development of internalising monoclonal antibodies to deliver toxic payloads directly to the cancer cells. Using immunohistochemistry, we screened various non\u2010cancerous and cancerous tissue sections to assess PTGFRN expression levels. We produced hybridoma lines that produce fully human antibodies against the PTGFRN extracellular domain. After screening, we conjugated the cytotoxic payload Duocarmycin to an antibody candidate and tested its efficacy in in\u00a0vitro assays, as well as in\u00a0vivo xenografted athymic nude mice. We showed that PTGFRN expression was undetectable in non\u2010cancerous tissue samples and overexpressed in several patient\u2010derived cancer tissue samples. We produced a hybridoma line that produces a fully human IgG1 (8C7) against PTGFRN. 8C7 binds to cell\u2010surface PTGFRN, inducing endocytosis of PTGFRN. Direct conjugation of Duocarmycin to 8C7 resulted in an antibody\u2010drug conjugate that showed high potency in in\u00a0vitro and in\u00a0vivo models for three PTGFRN\u2010expressing cell lines examined, A431, DAOY, and MSTO, while it had no effect on PTGFRN\u2010negative MDA\u2010MB\u2010231. 8C7\u2010ADC administered via intraperitoneal injection to xenografted mice showed inhibition of tumour formation and growth with no effect on body weight and organ weights. These findings further validate PTGFRN as a target for antibody\u2010drug conjugate development for cancers with unmet needs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcmm.70665","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T04:08:28Z","timestamp":1750738108000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>8C7<\/scp>: A Fully Human Anti\u2010<scp>PTGFRN<\/scp> Monoclonal Antibody\u2010Drug Conjugate Inhibiting Tumour Growth of Mesothelioma and Paediatric Medulloblastoma Cell Lines"],"prefix":"10.1111","volume":"29","author":[{"given":"Jorge","family":"Marquez","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Maryland, Baltimore School of Pharmacy  Baltimore Maryland USA"},{"name":"Target Discovery Division A&amp;G Pharmaceutical, Inc.  Columbia Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Dong","sequence":"additional","affiliation":[{"name":"Target Discovery Division A&amp;G Pharmaceutical, Inc.  Columbia Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binbin","family":"Yue","sequence":"additional","affiliation":[{"name":"Target Discovery Division A&amp;G Pharmaceutical, Inc.  Columbia Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Precision Antibody  Columbia Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Dong","sequence":"additional","affiliation":[{"name":"Precision Antibody  Columbia Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Oshimura","sequence":"additional","affiliation":[{"name":"Trans Chromosomics, Inc. 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