{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:47:27Z","timestamp":1792648047142,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 82303686"],"award-info":[{"award-number":["No. 82303686"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The global increase in thyroid cancer prevalence, particularly among female populations, underscores critical gaps in our understanding of molecular pathogenesis and diagnostic capabilities. Our investigation addresses these knowledge deficits by examining molecular signatures and validating diagnostic markers using clinical specimens to facilitate earlier detection and targeted therapeutic development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted comprehensive analyses of thyroid cancer specimens through multiple methodologies. Quantitative PCR and ELISA techniques were employed to quantify gene expression profiles and cytokine concentrations. High\u2010resolution single\u2010cell transcriptomics illuminated cellular communications within the tumour ecosystem, with particular emphasis on myeloid cell interactions mediated by MIF and GALECTIN signalling networks. Rigorous statistical frameworks were implemented to evaluate differential expression patterns and cytokine alterations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our analyses demonstrated pronounced elevation of both pro\u2010inflammatory mediators (TNF\u2010\u03b1, IL\u20106, IL\u20108, VEGF) and immunoregulatory cytokines (TGF\u2010\u03b2, IL\u201010) in neoplastic tissues relative to non\u2010malignant adjacent regions, with magnitude changes of 2.5\u20134.0 fold (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Network analysis revealed distinctive gene modules, notably MEblue and MEmagenta, exhibiting strong positive correlations with disease progression. Computational diagnostic algorithms, particularly penalised regression models (Ridge, Lasso), exhibited exceptional discriminatory capacity, achieving 0.963 AUC in external validation (GSE27155 dataset). Single\u2010cell profiling uncovered extensive communication networks centred on myeloid cell populations, with MIF and GALECTIN pathways emerging as critical mediators of tumour development and immune suppression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings expand the molecular understanding of thyroid carcinogenesis, highlighting the significance of myeloid\u2010centered communication networks. The molecular signatures and gene modules identified represent promising candidates for diagnostic applications and personalised therapeutic targeting. Prospective validation in expanded and heterogeneous patient populations remains essential to confirm clinical utility and optimise implementation strategies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcmm.70676","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T23:55:43Z","timestamp":1751414143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Global Thyroid Cancer Patterns and Predictive Analytics: Integrating Machine Learning for Advanced Diagnostic Modelling"],"prefix":"10.1111","volume":"29","author":[{"given":"Yao","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology Tianjin Medical University Cancer Institute &amp; Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongsheng","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Thyroid and Neck Oncology Tianjin Medical University Cancer Institute &amp; Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Tianjin Medical University Cancer Institute &amp; Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Thyroid and Neck Oncology Tianjin Medical University Cancer Institute &amp; Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiguo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Tianjin Medical University Cancer Institute &amp; Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3134-7790","authenticated-orcid":false,"given":"Zhiyong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Tianjin Medical University Cancer Institute &amp; Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201061075\u20106"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/ERC\u201024\u20100120"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.707233"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2023.1748"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph21060667"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2024.1367818"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2024.168654"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010024\u201003367\u20108"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.22037\/aaem.v12i1.2257"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(24)00079\u20103"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1401496"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics14121290"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201039329\u20106"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.060286"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2024.14636"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.10213"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2024.104720"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.685"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112891"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.CIR\u201023\u20100205"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2024.111093"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201023\u20102390"},{"issue":"6","key":"e_1_2_10_24_1","first-page":"2517","article-title":"Galectin\u20108 Alters Immune Microenvironment and Promotes Tumor Progression","volume":"13","author":"Wang Y.","year":"2023","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202306059"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010235"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70676","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T11:10:04Z","timestamp":1756293004000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":25,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/jcmm.70676"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70676","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2024-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70676"}}