{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:17:05Z","timestamp":1778624225957,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study aimed to elucidate the causal interplay between dietary habits, gut microbiota composition, circulating metabolites, serum proteins, laboratory biomarkers and kidney stone formation, employing Mendelian randomisation (MR) to identify potential mediators. A rigorous two\u2010sample MR framework was employed to assess the causal associations between kidney stones and a spectrum of predisposing factors. This encompassed dietary patterns, gut microbiota profiles, circulating metabolic intermediates, serum proteins and laboratory test indicators. Significant associations were further analysed using mediation analysis to uncover indirect pathways. Initial significance was determined at <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, followed by the implementation of False Discovery Rate correction (FDR <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) to reduce the likelihood of false positives due to multiple comparisons. Direct causal relationships were established between kidney stones and 9 dietary factors (including fruit, alcohol, coffee intake), 11 gut microbiota types, 8 metabolites, 12 plasma proteins and 8 laboratory indicators (CRE, EGFR, CA, UAHDL, APOA, CYS and URNA). Notably, nine mediation pathways were discovered. These pathways reveal the indirect effects of dietary habits on kidney stone formation mediated through laboratory biomarkers. Specifically, five dietary habits\u2014alcohol, coffee, fruit, champagne\/white wine and dried fruit consumption\u2014were shown to mediate through seven key factors: APOA, CA, CYS, EGFR, HDL, UA and URNA. Six of these mediations were positive, indicating facilitatory roles, while three exhibited negative mediation, suggestive of competitive inhibition in the diet\u2010kidney stone causal pathway. This MR study underscored the causal links between dietary habits, gut microbiota composition, circulating metabolites, serum proteins, laboratory biomarkers and kidney stone development, shedding light on potential mediators including seven laboratory biomarkers.<\/jats:p>","DOI":"10.1111\/jcmm.70698","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T12:59:24Z","timestamp":1752757164000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Causal Relationships Between Dietary Habits, Gut Microbiota, Metabolites, Serum Proteins and Laboratory Biomarkers in Kidney Stone Formation: A Mendelian Randomisation Study"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3955-5527","authenticated-orcid":false,"given":"Rongjiang","family":"Wang","sequence":"first","affiliation":[{"name":"The Department of Urology The First Afliated Hospital of Huzhou Normal College  Zhejiang China"},{"name":"Huzhou Key Laboratory of Precise Diagnosis and Treatment of Urinary Tumors  Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengting","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Department of Urology The First Afliated Hospital of Huzhou Normal College  Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaojun","family":"Li","sequence":"additional","affiliation":[{"name":"The Department of Urology The First Afliated Hospital of Huzhou Normal College  Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changbao","family":"Xu","sequence":"additional","affiliation":[{"name":"The Department of Urology The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Liang","sequence":"additional","affiliation":[{"name":"The Department of Urology Xinchang County Traditional Chinese Medicine Hospital  Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9077-6299","authenticated-orcid":false,"given":"Junwen","family":"Shen","sequence":"additional","affiliation":[{"name":"The Department of Urology The First Afliated Hospital of Huzhou Normal College  Zhejiang China"},{"name":"Huzhou Key Laboratory of Precise Diagnosis and Treatment of Urinary Tumors  Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Zhong","sequence":"additional","affiliation":[{"name":"The Department of Urology The First Afliated Hospital of Huzhou Normal College  Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-018-1090-6"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2020.09.011"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.16179"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12894-022-01017-4"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-020-0320-7"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016010098"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2009.812"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010162"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-021-00991-z"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-020-00747-1"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07148-y"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-022-01270-1"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xgen.2024.100506"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-020-00757-z"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05473-8"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/HEP.0000000000000649"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00092-5"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2022-327860"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a039586"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-021-00757-1"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(23)00086-4"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyx034"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13061917"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-020-03466-8"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/end.2020.0378"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e32836214b9"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000484286"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.134981"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a040501"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2023.03.029"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70698","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T07:10:32Z","timestamp":1753859432000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70698"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":30,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/jcmm.70698"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70698","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70698"}}