{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:08:00Z","timestamp":1787015280826,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T00:00:00Z","timestamp":1751760000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82004392"],"award-info":[{"award-number":["82004392"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Glucocorticoid\u2010induced osteoporosis is a common clinical orthopaedic disease, primarily due to excessive use of hormones. Tectorigenin has shown a certain efficacy in treating osteoporosis, but its underlying mechanisms are not yet fully understood. This study aimed to evaluate tectorigenin (TEC)'s potential in treating glucocorticoid\u2010induced osteoporosis (GIOP) by restoring the osteoclast\u2013osteoblast balance. By employing molecular docking to study TEC\u2013RANKL interaction and RNA sequencing to map TEC's anti\u2010osteoporotic pathways, we conducted in\u00a0vitro tests on TEC's effects on osteoclast differentiation, bone resorption, ROS production, and NF\u2010\u03baB activation. We performed a luciferase reporter assay for Nrf2 and NF\u2010\u03baB activities and evaluated TEC's in\u00a0vivo efficacy using a GIOP mouse model with micro\u2010CT and histomorphometric analyses, measuring serum RANKL levels and Treg\/Th17 cell ratios. Docking analysis confirmed TEC's specific binding to RANKL and RNA sequencing showed TEC modulated osteoclast pathways. In\u00a0vitro, TEC suppressed osteoclastogenesis, bone resorption, osteoclast gene expression, and ROS activity by downregulating NFATc1 and modulating NF\u2010\u03baB. The luciferase assay revealed TEC inhibited Nrf2 and influenced its interaction with NF\u2010\u03baB. In\u00a0vivo, TEC protected against GIOP by balancing Th17\/Treg cells to inhibit osteoclast differentiation and maintain bone volume. In conclusion, tectorigenin (TEC) shows promise in reducing osteoclastogenesis and preventing GIOP, making it a potential drug candidate for osteoporosis management.<\/jats:p>","DOI":"10.1111\/jcmm.70705","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T01:17:30Z","timestamp":1751851050000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tectorigenin Ameliorates Glucocorticoid\u2010Induced Osteoporosis by Inhibiting the\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    Signal Pathway and Modulating Treg\u2010Th17 Cell Balance"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4226-6248","authenticated-orcid":false,"given":"Peng","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Orthopedics Guangdong Provincial Second Hospital of Traditional Chinese Medicine  Guangzhou China"},{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome  Guangzhou China"},{"name":"Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puiian","family":"Wong","sequence":"additional","affiliation":[{"name":"Faculty of Chinese Medicine Macau University of Science and Technology  Macau China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Lv","sequence":"additional","affiliation":[{"name":"The First People's Hospital of Zhaoqing  Zhaoqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqing","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Guangdong Provincial Second Hospital of Traditional Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[{"name":"Guangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine  Guangzhou Guangdong China"},{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome\/Orthopaedic The Third Affiliated Hospital of Guangzhou University of Chinese Medicine  Guangzhou Guangdong China"},{"name":"Department of Orthopaedics, the Third Affiliated Hospital Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushi","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine  Guangzhou Guangdong China"},{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome\/Orthopaedic The Third Affiliated Hospital of Guangzhou University of Chinese Medicine  Guangzhou Guangdong China"},{"name":"Department of Orthopaedics, the Third Affiliated Hospital Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Mo","sequence":"additional","affiliation":[{"name":"Macao Health Bureau WHO Traditional Medicine Cooperation Center  Macau China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mincong","family":"He","sequence":"additional","affiliation":[{"name":"Guangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine  Guangzhou Guangdong China"},{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome\/Orthopaedic The Third Affiliated Hospital of Guangzhou University of Chinese Medicine  Guangzhou Guangdong China"},{"name":"Department of Orthopaedics, the Third Affiliated Hospital Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-016-3791-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-020-0341-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-017-0335-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198308043090502"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2016.07.043"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0459"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300213200"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1997.12.5.734"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2869"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-020-01162-6"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S225516"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.18.2412"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/16852"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.9.7634"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2855"},{"key":"e_1_2_9_17_1","first-page":"1130","article-title":"Chen, Anti\u2010Inflammatory and Anti\u2010Osteoarthritis Effects of Tectorigenin","volume":"6","author":"Wang C. L.","year":"2017","journal-title":"Biology Open"},{"issue":"5","key":"e_1_2_9_18_1","first-page":"476","article-title":"Tectorigenin Promotes Osteoblast Differentiation and In\u00a0Vivo Bone Healing, but Suppresses Osteoclast Differentiation and In\u00a0Vivo Bone Resorption","volume":"41","author":"Lee S. Y.","year":"2018","journal-title":"Molecules and Cells"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13801"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28513"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8363295"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S435301"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41232-019-0111-3"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.5152\/eurjrheum.2016.048"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540405200312"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18010203"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1197-1285"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2021.115449"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6299"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00657"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/35046196"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.2741\/4600"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34565"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-02067-3"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-024-00910-6"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.177841"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2009.06.030"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034647"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70705","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T11:10:27Z","timestamp":1756293027000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":37,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/jcmm.70705"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70705","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2024-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70705"}}