{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T01:54:34Z","timestamp":1785549274505,"version":"3.56.0"},"reference-count":72,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Periodontitis (PD) and primary Sj\u00f6gren's syndrome (pSS) are persistent inflammatory diseases that affect oral health, and numerous studies have uncovered phenotypic and intrinsic correlations between the two. However, a comprehensive and high\u2010resolution analysis at the single\u2010cell level has not been reported comparing the immune cell landscapes in PD and pSS. This study integrated single\u2010cell transcriptome data from peripheral blood mononuclear cells (PBMCs) of healthy controls (HC), PD and pSS patients sourced from the NCBI and NGDC databases. The scRNA datasets were integrated, and cells were clustered using Seurat, with further subdivision of T\/NK, myeloid cells and B cell subclusters. Next, NicheNetr was used to analyse the communication between myeloid cells and CD8<jats:sup>+<\/jats:sup> naive T (Tn) cells. Finally, we identified nine T\/NK cell subclusters, seven myeloid cell subclusters and five B cell subclusters. Compared to the HC group, the proportion of CD8<jats:sup>+<\/jats:sup> Tn cells was significantly downregulated in both PD and pSS. HLA\u2010DRA<jats:sup>+<\/jats:sup> mono cells showed a significant decrease in PD. Additionally, immature B cells were upregulated in PD but showed an opposite trend in pSS. The intracellular communication analysis between myeloid cells and CD8<jats:sup>+<\/jats:sup> Tn cells suggested that myeloid cells may promote the exhaustion of CD8<jats:sup>+<\/jats:sup> Tn cells through the <jats:italic>IL\u20107\/TNFSF11\u2010CDKN1A<\/jats:italic> signalling pathways. The <jats:italic>BACE2\u2010CDKN1A<\/jats:italic> signalling pathway may also contribute to the depletion of CD8<jats:sup>+<\/jats:sup> Tn cells in both PD and pSS. Collectively, this study uncovers the single\u2010cell level associations between PD and pSS, providing new insights into the common molecular mechanisms underlying these two diseases.<\/jats:p>","DOI":"10.1111\/jcmm.70713","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T03:18:19Z","timestamp":1753240699000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Featured Immune Characteristics of Periodontitis and Primary Sj\u00f6gren's Syndrome Revealed by Single\u2010Cell Transcriptome Analyses"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1175-3991","authenticated-orcid":false,"given":"Juanjuan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Stomatology, Ninth Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Ninth Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Aviation General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Ninth Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianyu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Ninth Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Ninth Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuhua","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Ninth Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(15)60692\u20104"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010030\u201096881\u20106_8"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/idj.12630"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis\u20102016\u2010210448"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10238\u2010021\u201000728\u20106"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584\u2010023\u201000932\u20106"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.23736\/S0026\u20104784.20.04494\u20109"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2022.08.001"},{"issue":"6","key":"e_1_2_10_10_1","first-page":"365","article-title":"Sjogren's Syndrome: Oral Manifestations and Treatment, a Dental Perspective","volume":"89","author":"Cartee D. 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