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In this study, we investigated the role of NPF\u2010related genes in cancer prognosis by analysing gene expression and clinical data from the TCGA pan\u2010cancer dataset. A multigene prognostic model was developed to predict patient survival. Pathway enrichment and tumour microenvironment analyses revealed significant associations between NPF\u2010related genes and immune cell types, particularly highlighting the link between <jats:italic>TLR4<\/jats:italic> expression and macrophage infiltration. Elevated <jats:italic>TLR4<\/jats:italic> expression in tumour cells was correlated with an immunosuppressive environment, positioning <jats:italic>TLR4<\/jats:italic> as a potential therapeutic target. Moreover, treatment with the <jats:italic>TLR4<\/jats:italic> inhibitor TAK\u2010242 was shown to inhibit cell proliferation and migration in PANC\u20101 and SW1990 cell lines. These findings underscore the potential of NPF\u2010related pathways as prognostic biomarkers and support TAK\u2010242 as a promising therapeutic candidate for cancer treatment.<\/jats:p>","DOI":"10.1111\/jcmm.70742","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T03:39:07Z","timestamp":1753241947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pan\u2010Cancer Analyses of Necroptosis, Pyroptosis and Ferroptosis Related Genes Reveal <scp>TLR4<\/scp> as A Potential Therapeutic Target"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3179-1719","authenticated-orcid":false,"given":"Yu","family":"Shen","sequence":"first","affiliation":[{"name":"Biomedical Big Data Center, the First Affiliated Hospital Zhejiang University School of Medicine  Zhejiang China"},{"name":"Center of Regenerative Medicine, Zhejiang University School of Medicine 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