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In this study, we investigated the role of N6\u2010methyladenosine (m6A) modification and its regulatory factors, METTL3 and METTL14, in GBC. Our results showed that m6A levels as well as the expression of METTL3 and METTL14 were significantly downregulated in GBC tissues compared to normal gallbladder tissues. In\u00a0vitro experiments showed that manipulation of METTL3 and METTL14 expression regulated GBC cell migration and invasive ability, and the liver metastasis model of nude mice further demonstrated the involvement of m6A modification in regulating GBC metastasis. Further investigation identified miR\u2010146a\u20105p as a downstream target regulated by m6A, with tumour\u2010suppressive effects on GBC cell migration and invasion. Overall, our findings provide new insights into the role of m6A modification and its regulation on microRNA in GBC pathogenesis and offer a potential strategy for the treatment of GBC.<\/jats:p>","DOI":"10.1111\/jcmm.70764","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T03:44:28Z","timestamp":1754883868000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dysregulated\n                    <scp>RNA m6A<\/scp>\n                    Methylation Contributes to the Metastasis of Gallbladder Cancer Through\n                    <scp>miR<\/scp>\n                    \u2010146a\u20105p"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3073-2523","authenticated-orcid":false,"given":"Yuhui","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Biliary\u2010Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College Huazhong 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