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Clinical samples from 30 AVS patients and 30 controls treated at Tianjin Chest Hospital between 2010 and 2020 were collected. Analyses included immunofluorescence detection, Western blotting (WB), quantitative RT\u2010PCR analysis (qRT\u2010PCR), haematoxylin and eosin (HE) staining and molecular docking experiments of protein\u2013protein interactions. USP14 was found to be highly expressed in AVS tissues, which was validated by immunofluorescence and WB analyses. qRT\u2010PCR results indicated that the mRNA expression levels of USP14 and CDK4 were significantly elevated in AVS tissues. HE staining revealed significant pathological changes in AVS tissues. Molecular docking experiments demonstrated the interaction between USP14 and CDK4, suggesting a potential regulatory mechanism in AVS. USP14 may be involved in the occurrence and development of AVS by regulating cell proliferation, apoptosis and fibrosis processes. It may serve as a therapeutic target for treating AVS.<\/jats:p>","DOI":"10.1111\/jcmm.70765","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T04:17:09Z","timestamp":1758687429000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>USP14<\/scp>\n                    Mediates Molecular Mechanisms Regulating Aortic Valve Stenosis Through Ubiquitination"],"prefix":"10.1111","volume":"29","author":[{"given":"Yin","family":"Yang","sequence":"first","affiliation":[{"name":"Cardiovascular Surgery Tianjin Medical University, Tianjin Chest Hospital  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo\u2010Chen","family":"Yao","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Tianjin Medical University, Tianjin Chest Hospital  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Tianjin Medical University, Tianjin Chest Hospital  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing\u2010Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Tianjin Medical University, Tianjin Chest Hospital  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Tianjin Medical University, Tianjin Chest Hospital  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lian\u2010Qun","family":"Wang","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Tianjin Medical University, Tianjin Chest Hospital  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4956-3213","authenticated-orcid":false,"given":"Zhi\u2010Gang","family":"Guo","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Tianjin Medical University, Tianjin Chest Hospital  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2012-302265"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2018.01.020"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ehjci\/jex211"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamacardio.2016.2060"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2016.10.005"},{"issue":"1","key":"e_1_2_12_7_1","first-page":"G113","article-title":"Functional Somatostatin Receptors on a Rat Pancreatic Acinar Cell Line","volume":"255","author":"Viguerie N.","year":"1988","journal-title":"American Journal of Physiology"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09299"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2015.4296"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-017-0069-z"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.04.022"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2013.28"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000443051"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102231"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.6"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.08.988"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2010.202"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2014.03.022"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.02.093"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2004.08.013"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2023.1119746"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-014-1229-z"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9090472"},{"issue":"5","key":"e_1_2_12_24_1","first-page":"371","article-title":"Aortic Stenosis: Diagnosis and Treatment","volume":"93","author":"Grimard B. 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