{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T09:00:31Z","timestamp":1769331631667,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018555","name":"Science and Technology Program of Guizhou Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018555","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Although the pathogenesis of Perthes disease remains unclear, the mechanism of bone regeneration in the defective femoral head is an area of particular interest. Improving understanding of the underlying mechanisms is essential for the development of an effective treatment for this condition. This study explored the roles of secreted frizzled\u2010related protein 2 (SFRP2) and micro ribonucleic acid (miR)\u2010106a\u20105p in the regulation of osteogenic differentiation (in vitro) and bone regeneration (in vivo). Cells were transfected with plasmids carrying the genes encoding SFRP2 and miR\u2010106a\u20105p. Cell proliferation and apoptosis were evaluated using 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine staining, flow cytometry and the terminal deoxynucleotidyl transferase dUTP nick end\u2010labelling assay. Osteogenic differentiation was identified using alkaline phosphatase and alizarin red S staining. Reverse transcription quantitative polymerase chain reaction and western blot were used to assess messenger ribonucleic acid and protein levels. The dual\u2010luciferase reporter gene assay was used to confirm the targeting relationship between miR\u2010106a\u20105p and SFRP2. Changes in bone structure were evaluated by morphological observation, micro\u2010computed tomography and alkaline phosphatase staining. The findings showed that SFRP2 significantly increased cell proliferation and osteogenic differentiation and inhibited apoptosis of bone marrow mesenchymal stem and MC3T3\u2010E1 cells. It upregulated the expression of PCNA, Bcl\u20102, ALP, Col1a1, Runx2, Osterix, Wnt3a, \u03b2\u2010catenin, LRP5 and LRP6, and downregulated that of Bax. Negative regulation of SFRP2 by miR\u2010106a\u20105p (via the Wnt\/\u03b2\u2010catenin pathway) could rescue the influence of the latter; this showed that SFRP2 is a target gene of miR\u2010106a\u20105p.In conclusion, miR\u2010106a\u20105p\/SFRP2 may play a crucial role in bone regeneration in the defective femoral head and may be a potential therapeutic target in Perthes disease.<\/jats:p>","DOI":"10.1111\/jcmm.70804","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T03:54:38Z","timestamp":1758513278000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Secreted Frizzled\u2010Related Protein 2 Promotes Osteogenic Differentiation and Bone Regeneration in Perthes Disease When Targeted by\n                    <scp>miR<\/scp>\n                    \u2010106a\u20105p"],"prefix":"10.1111","volume":"29","author":[{"given":"Tianjiu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery, Guizhou Children's Hospital Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Clinical Medicine Guizhou Medical University  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiafei","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine Guizhou Medical University  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Guizhou Children's Hospital Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Clinical Medicine Guizhou Medical University  Guiyang 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