{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T11:03:23Z","timestamp":1779707003740,"version":"3.53.1"},"reference-count":45,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["DST\/INSPIRE\/04\/2015\/001777"],"award-info":[{"award-number":["DST\/INSPIRE\/04\/2015\/001777"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["3\/1\/3(18)\/Endo\u2010fellowship\/22\u2010NCD\u2010III"],"award-info":[{"award-number":["3\/1\/3(18)\/Endo\u2010fellowship\/22\u2010NCD\u2010III"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020217","name":"Sanjay Gandhi Postgraduate Institute of Medical Sciences","doi-asserted-by":"publisher","award":["A\u201019\u2010PGI\/IMP\/86\/2022"],"award-info":[{"award-number":["A\u201019\u2010PGI\/IMP\/86\/2022"]}],"id":[{"id":"10.13039\/100020217","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>IL10<\/jats:italic>\n                    is a very effective anti\u2010inflammatory cytokine.\n                    <jats:italic>IL10<\/jats:italic>\n                    imbalance is linked to type 2 diabetes mellitus (T2DM) and also to renal hypertrophy, glomerular membrane thickening, and onset of diabetic nephropathy (DN). We aimed to investigate the association of\n                    <jats:italic>IL10<\/jats:italic>\n                    gene polymorphism (rs1800871T\/C, rs1800896A\/G) with DN and determine the influence of variants on its expression level and interaction with transcription factors. We genotyped 301 study subjects, comprising 75 DN, 126 T2DM patients, and 100 controls. All were analysed for biochemical assays and genotypic analysis by PCR\u2010RFLP and confirmed by Sanger sequencing. The haplotype analysis was calculated by Chi\u2010square test. mRNA expression and its correlation with variants were assessed by using RT\u2010PCR. Statistical analyses were done by using SPSS and GraphPad software. Screening of transcription factors with\n                    <jats:italic>IL10<\/jats:italic>\n                    gene variants was performed using the TRANSFAC database, and the variants impact on\n                    <jats:italic>IL10<\/jats:italic>\n                    molecular interactions was analysed. This study revealed that\n                    <jats:italic>IL10<\/jats:italic>\n                    gene polymorphism rs1800896 was significantly associated with DN. \u2018CG\u2019 and \u2018TG\u2019 haplotypes were significantly associated with DN. Expression levels of\n                    <jats:italic>IL10<\/jats:italic>\n                    were upregulated in DN patients. The genetic correlation study shows that\n                    <jats:italic>IL10<\/jats:italic>\n                    gene expression was downregulated in the rs1800871 alternate variant genotype (CC) while upregulated in the rs1800896 alternate variant genotype (GG).\n                    <jats:italic>In silico<\/jats:italic>\n                    analyses suggest that the binding affinity of transcription factor CEBPA decreases due to the rs1800871 alternate variant, while the affinity of KLF4 increases in the case of the rs1800896 alternate variant. Our\n                    <jats:italic>in silico<\/jats:italic>\n                    results correlated with\n                    <jats:italic>IL10<\/jats:italic>\n                    expression analysis in respective patient groups. Overall, our findings highlight the role of\n                    <jats:italic>IL10<\/jats:italic>\n                    gene polymorphism in DN progression in the North Indian population.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70819","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T04:35:19Z","timestamp":1757651719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Prognostic Significance of\n                    <scp>\n                      <i>IL10<\/i>\n                    <\/scp>\n                    Variants and Their Mechanistic Regulation in Diabetic Nephropathy"],"prefix":"10.1111","volume":"29","author":[{"given":"Neha","family":"Shukla","sequence":"first","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Kumari","sequence":"additional","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poornima","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amisha","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyan Manjary","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narayan","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Nephrology Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushil","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshika","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. S.","family":"Ansari","sequence":"additional","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soorya","family":"Janakiraman","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Bioinformatics University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marey","family":"Baden","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Bioinformatics University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Wolkenhauer","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Bioinformatics University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailendra K.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Bioinformatics University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8644-4344","authenticated-orcid":false,"given":"Naveen Kumar","family":"Gautam","sequence":"additional","affiliation":[{"name":"Department of Urology and Renal Transplantation Sanjay Gandhi Post Graduate Institute of Medical Sciences  Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,11]]},"reference":[{"issue":"1","key":"e_1_2_12_2_1","first-page":"49","article-title":"Diabetic Kidney Disease: World Wide Difference of Prevalence and Risk Factors","volume":"5","author":"Gheith O.","year":"2016","journal-title":"Journal of 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