{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:05:30Z","timestamp":1789578330589,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Osteoarthritis (OA) is a degenerative joint disease characterised by cartilage breakdown, leading to pain and reduced quality of life. This study aims to investigate the therapeutic potential of Puerarin (PUE), a natural compound derived from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pueraria lobata<\/jats:italic>\n                    <\/jats:styled-content>\n                    , in modulating OA progression. Employing a multifaceted approach that included bioinformatics analysis, molecular docking, in\u00a0vitro assays, and in\u00a0vivo experiments, we identified 57 overlapping targets between PUE and OA\u2010related genes, suggesting a multi\u2010target interaction model. Our findings revealed that PUE effectively inhibited inflammatory cytokine production and protected chondrocytes from apoptosis at non\u2010cytotoxic concentrations (5 and 10\u2009\u03bcM), promoting extracellular matrix synthesis by reversing IL\u20101\u03b2\u2010induced degradation of Aggrecan and Collagen II while reducing MMP\u201013 and ADAMTS5 expression. Furthermore, PUE was shown to attenuate IL\u20101\u03b2\u2010induced apoptosis by restoring BCL\u20102 levels and decreasing cleaved caspase\u20103 levels. In\u00a0vivo, PUE administration in a destabilisation of the medial meniscus (DMM) mouse model significantly slowed OA progression, preserving cartilage structure and reducing osteophyte formation. Moreover, PUE activated the PI3K\u2010AKT signalling pathway, underscoring its anti\u2010inflammatory and anti\u2010apoptotic mechanisms. Collectively, these results support Puerarin's potential as a disease\u2010modifying agent in OA treatment, warranting further clinical exploration and consideration of its application in combination therapies to enhance cartilage protection and repair.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70833","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T10:48:42Z","timestamp":1758797322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Puerarin Attenuates Osteoarthritis via Multi\u2010Target Regulation of Inflammation, Apoptosis, and\n                    <scp>ECM<\/scp>\n                    Degradation"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0527-8513","authenticated-orcid":false,"given":"Yanjing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, The Eighth Affiliated Hospital Southern 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