{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:32:22Z","timestamp":1780677142422,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T00:00:00Z","timestamp":1757030400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The important role of the EphrinB2\u2013EphB4 signalling pathway in bone remodelling has been demonstrated, while its effect on inflammatory bone defect regeneration remains poorly understood. This study was to assess the effect of EphB4\u2013EphrinB2 signalling on inflammation\u2010mediated bone defect repair in murine models. The modelling method of inflammation\u2010mediated bone defect in mice was established by intraperitoneally injecting different concentrations of TNF\u2010\u03b1. Then, three randomly assigned groups were administered vehicle (PBS, control), EphrinB2 siRNA, and EphB4 siRNA into a 1.5\u2010mm diameter mandibular bone defect with 5\u2009\u03bcg\/kg TNF\u2010\u03b1 intraperitoneally injected every 2\u2009days. The gene expression of osteogenic differentiation markers Runx2, Osterix, ALP, OCN and BSP in healing tissue of the bone defect was examined by quantitative real\u2010time polymerase chain reaction (PCR). Runx2 and BSP protein expressions were examined by western blot, and the decalcified tissues were subjected to histological examination. Compared with the control group, the EphB4 siRNA group mice exhibited lower levels of osteogenic differentiation markers and higher levels of the osteoclastogenic marker. H&amp;E staining, TRACP staining and bone histomorphometry showed that the bones were thinner and the number of giant osteoclasts in the EphB4 siRNA group was higher compared with the control group, whereas there were no significant differences in osteoblastic and osteoclastic differentiation between EphrinB2 siRNA mice and control mice. In conclusion, the EphrinB2\u2013EphB4 signalling pathway plays a critical role in the inflammation\u2010induced bone defect repair process; selective inhibition of EphB4 using siRNA results in decreased bone formation and increased bone resorption under high inflammatory circumstances in\u00a0vivo.<\/jats:p>","DOI":"10.1111\/jcmm.70840","type":"journal-article","created":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T04:13:27Z","timestamp":1757132007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Role of\n                    <scp>EphrinB2<\/scp>\n                    \u2013\n                    <scp>EphB4<\/scp>\n                    Signalling Pathway in Regeneration of Inflammatory Bone Defect"],"prefix":"10.1111","volume":"29","author":[{"given":"Lili","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Stomatology Liaocheng People's Hospital  Liaocheng Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Stomatology Liaocheng People's Hospital  Liaocheng Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Liaocheng People's Hospital  Liaocheng Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9847-8624","authenticated-orcid":false,"given":"Rongjing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Stomatology Liaocheng People's Hospital  Liaocheng Shandong People's Republic of 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