{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T13:38:04Z","timestamp":1770471484105,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2025AFD794"],"award-info":[{"award-number":["2025AFD794"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31971166"],"award-info":[{"award-number":["31971166"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ginsenoside Rg5 (G\u2010Rg5), a rare extract of ginseng, has proven to be valuable for a wide range of clinical applications. However, the role of G\u2010Rg5 in radiation\u2010induced heart injury is currently unclear. This study aims to explore the intervention effect and possible treatment mechanism of G\u2010Rg5 on radiation\u2010induced heart injury. We investigated the impact of G\u2010Rg5 on radiation\u2010induced heart injury through in\u00a0vivo and in\u00a0vitro studies. Network pharmacology was employed to explore potential key targets and molecular mechanisms. Various experimental methods were utilised to validate the effects of G\u2010Rg5 on the PPARG\/PDK1\/AKT1 pathway. G\u2010Rg5 alleviated radiation\u2010induced cardiomyocyte apoptosis and cardiac functional impairment. The expression of peroxisome proliferator activated receptor gamma (PPARG) was upregulated following G\u2010Rg5 treatment, thereby suppressing the transcription of phosphoinositide\u2010dependent protein kinase 1 (PDK1), a predicted target gene regulated by PPARG, to enhance AKT serine\/threonine kinase 1 (AKT1) phosphorylation levels. The protective effect of G\u2010Rg5 against radiation\u2010induced heart injury was found to be compromised upon inhibition or knockdown of PPARG. The interaction between PPARG and PDK1 was confirmed by the results of chromatin immunoprecipitation and luciferase reporter assays. G\u2010Rg5, through the upregulation of PPARG expression, induces the transcription of PDK1 and subsequently enhances the phosphorylation levels of AKT1. Ultimately, this process plays a crucial role in mitigating cellular apoptosis and functional decline in radiation\u2010induced heart injury. Therefore, G\u2010Rg5 holds great potential as a therapeutic agent for radiation\u2010induced heart injury.<\/jats:p>","DOI":"10.1111\/jcmm.70852","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T05:00:31Z","timestamp":1760590831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ginsenoside Rg5 Improves Radiation\u2010Induced Heart Injury via\n                    <scp>PPARG<\/scp>\n                    \/\n                    <scp>PDK1<\/scp>\n                    \/\n                    <scp>AKT1<\/scp>\n                    Pathway"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9715-293X","authenticated-orcid":false,"given":"Dao\u2010ming","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Oncology Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010jian","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Oncology Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao\u2010yue","family":"Li","sequence":"additional","affiliation":[{"name":"CCU Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Oncology Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, School of Life Sciences Jilin University  Changchun People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Oncology Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Oncology Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Oncology Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Renmin Hosptial of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi\u2010ming","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Oncology Renmin Hospital of Wuhan University  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3857\/roj.2021.00766"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3579143"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jrr\/rrw064"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2017-0121"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.5142\/jgr.2012.36.1.1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10272"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110408"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.43"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00392-021-01809-y"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnur.2020.10.011"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-018-0214-9"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13090236"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21743"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgr.2020.03.001"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39514-1"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-024-01231-w"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-020-00434-y"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/09603271241229140"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5040326"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgr.2023.06.004"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.14217"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01531-3"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-00714-7"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm12020243"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15061778"},{"key":"e_1_2_11_27_1","article-title":"Integrating Literature\u2010Based Knowledge Database and Expression Data to Explore Molecular Pathways Connecting PPARG and Myocardial Infarction","volume":"6","author":"Cao R.","year":"2020","journal-title":"PPAR Research"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.3113"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2011.01527.x"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-012-1501-x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/1074248417707049"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11233834"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20180334"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22413"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2021.10.015"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:54:42Z","timestamp":1761659682000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":34,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/jcmm.70852"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70852","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70852"}}