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A mouse model of cardiac hypertrophy was established via abdominal aortic coarctation surgery. An in\u00a0vitro cell model was generated using neonatal mouse ventricular cardiomyocytes (NMVCs) co\u2010cultured with angiotensin II. Differentially expressed circular RNAs (circRNAs) were identified using next\u2010generation sequencing, and potential target microRNAs (miRNAs) and downstream messenger RNAs (mRNAs) were predicted using bioinformatic analysis. Reverse transcription quantitative polymerase chain reaction and western blotting were used to evaluate the expression of myocardial\u2010associated molecules at the transcriptional and translational levels, respectively. Expression of regulatory molecules was assessed after transfection with small interfering RNAs (siRNAs) or co\u2010culture with miRNA mimics. Dual\u2010luciferase reporter assays were performed to validate interactions between circRNAs and miRNAs. circRftn1 expression was significantly elevated both in\u00a0vivo and in\u00a0vitro. Concurrently, the expression of miR\u20101192 was decreased, whereas its target mRNAs TRIM25 and TRIM41 were markedly upregulated. Knockdown of circRftn1 via siRNA transfection reversed cardiac hypertrophy and led to opposing expression trends of miR\u20101192 and TRIM25\/TRIM41. Dual\u2010luciferase reporter assays confirmed the sponge\u2010like interaction between circRftn1 and miR\u20101192. By co\u2010culturing NMVCs with miR\u20101192 mimics, its targets TRIM25\/TRIM41 showed significant decreases. Moreover, NF\u2010\u03baB signalling pathway appeared to be involved, as the expression of the p65 subunit paralleled that of circRftn1. circRftn1 may promote cardiac hypertrophy by modulating the miR\u20101192\u2010TRIM25\/TRIM41 axis and NF\u2010\u03baB p65 pathway potentially serving as a downstream pathway.<\/jats:p>","DOI":"10.1111\/jcmm.70892","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:36:32Z","timestamp":1760405792000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Circular <scp>RNA<\/scp> Rftn1 Promotes Cardiac Hypertrophy In\u00a0Vitro and In\u00a0Vivo by Sponging <scp>miRNA<\/scp>\u20101192 to Upregulate Tripartite Motif Protein 25 and 41"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8138-5930","authenticated-orcid":false,"given":"Guangcheng","family":"Liu","sequence":"first","affiliation":[{"name":"Department of 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