{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T08:22:54Z","timestamp":1764404574493,"version":"3.46.0"},"reference-count":41,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260137","32460142"],"award-info":[{"award-number":["32260137","32460142"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Pathological cardiac hypertrophy was an important inducement of heart failure, cardiac arrest, and other diseases. To explore how Vasn knockout induced pathological cardiac hypertrophy, bioinformatics and functional studies illustrated the possible mechanism by clarifying the influence of exosome miRNA on the p\u2010MLC2 signal pathway. B\u2010ultrasound, electrocardiogram, pathological staining, and Q\u2010PCR were used to clarify the changes in typical imaging indexes, pathological indexes, and marker molecules. Exosome sequencing and bioinformatics analysis were carried out to mine key miRNA and signal pathways. Q\u2010PCR, IHC, and WB were used to verify the changes in miRNA and related signal pathways. The changes in heart structure and function were detected by pathological staining, electron microscopy, B\u2010ultrasound, and blood biochemistry in the heart tissues and blood of Vasn knockout mice. Vasn knockout mice showed typical imaging, pathological, and molecular features of PCH. Differential analysis of exosome miRNA showed that let\u20107g\u20105p, let\u20107f\u20105p, and miR\u2010148a\u20103p significantly increased in the exosomes of Vasn\u2010knockout mice heart. Bioinformatics analysis showed that let\u20107g\u20105p and let\u20107f\u20105p targeted the Calm\/MLCK\/p\u2010MLC2 signal pathway, and miR\u2010148a\u20103p targeted the Rhoa\/ROCK1\/p\u2010MLC2 signal pathway. The expression levels of miRNA were significantly up\u2010regulated, but related proteins of signal pathways were significantly reduced in Vasn knockout mice. The structure and function showed obvious damage in Vasn knockout mice. VASN knockout led to pathological cardiac hypertrophy, which may regulate the p\u2010MLC2 signalling pathway through exosomal miRNA.<\/jats:p>","DOI":"10.1111\/jcmm.70929","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T10:26:39Z","timestamp":1763115999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Network of Exosomes\n                    <scp>miRNA<\/scp>\n                    and p\u2010\n                    <scp>MLC2<\/scp>\n                    Regulatory Pathway Induced Pathological Cardiac Hypertrophy in Vasn Deficient Mice"],"prefix":"10.1111","volume":"29","author":[{"given":"Bin","family":"Huang","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Youjiang Medical University for Nationalities  Baise Guangxi China"},{"name":"Key Laboratory of Research and Development on Clinical Molecular Diagnosis for High\u2010Incidence Diseases of Baise  Baise 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