{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T12:00:14Z","timestamp":1789041614113,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T00:00:00Z","timestamp":1762819200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sepsis frequently progresses to acute lung injury (ALI), characterised by inflammation, extracellular matrix degradation, and mitochondrial dysfunction. This study identifies\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    as a gut\u2010derived bacterium that exploits the host fibrinolytic system for pulmonary translocation, resulting in mitochondrial damage and exacerbating lung injury. Utilising the cecal ligation and puncture (CLP) mouse model combined with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    pulmonary infection, we demonstrated that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    exacerbates lung injury by activating fibrinolysis, disrupting intestinal barrier integrity, and impairing mitochondrial function. Key findings include elevated plasmin activity, increased fibrin degradation products (FDP), and reduced expression of tight junction proteins ZO\u20101 and occludin. Mitochondrial dysfunction was confirmed by disrupted ultrastructure, impaired ATP synthesis, and increased ROS levels. Histological analyses revealed severe alveolar damage, neutrophil infiltration, and edema. Treatment with the fibrinolysis inhibitor aminocaproic acid or the mitochondrial protector MitoTEMPO alleviated fibrinolytic activity, preserved mitochondrial function, and reduced lung damage. Notably, combination therapy showed the most significant protective effects, improving lung histology and decreasing inflammation markers. This study provides novel insights into sepsis\u2010induced lung injury, highlighting\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. faecalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    and the fibrinolytic system as potential therapeutic targets.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70937","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:47:10Z","timestamp":1762919230000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Enterococcus faecalis<\/i>\n                    Translocation in Sepsis: Fibrinolysis and Mitochondrial Dysfunction Drive Lung Injury"],"prefix":"10.1111","volume":"29","author":[{"given":"Chenfei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Emergency, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Emergency, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhui","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Emergency, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changqing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Emergency, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1598-7835","authenticated-orcid":false,"given":"Keji","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-021-01545-5"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2021.174522"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.683645"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S488655"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2024.117155"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000000242"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00581-17"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.168"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.984835"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01500-2021"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellin.2022.100047"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0041-1725096"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241512376"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241814179"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-001-1139-8"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1515\/jccm-2017-0011"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-024-07586-2"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010936"},{"key":"e_1_2_12_20_1","article-title":"Identification of Plasminogen\u2010Binding Sites in \n                     Streptococcus suis\n                   Enolase That Contribute to Bacterial Translocation Across the Blood\u2013Brain Barrier","volume":"14","author":"Zhao T.","year":"2024","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.214"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2018.04.016"},{"issue":"4","key":"e_1_2_12_23_1","first-page":"893","article-title":"Bleomycin\u2010Induced Interstitial Pulmonary Disease in the Nude, Athymic Mouse","volume":"120","author":"Szapiel S. 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