{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:32:06Z","timestamp":1788219126463,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Astragalus polysaccharides (APS) are a crucial bioactive component known for their various pharmacological properties. Abnormal O\u2010linked \u03b2\u2010N\u2010acetylglucosamine modification (O\u2010GlcNAcylation) is noted in cases of intervertebral disc degeneration (IVDD). Nonetheless, it remains uncertain whether APS regulates the process of O\u2010GlcNAcylation associated with IVDD. We employed molecular docking, cycloheximide chase assay, immunohistochemistry, and immunoprecipitation to investigate APS\u2010mediated OGT\/O\u2010GlcNAcylation regulation of Nrf2. The effects of APS and its role in promoting the O\u2010GlcNAcylation of Nrf2 in IVDD through both in\u00a0vivo and in\u00a0vitro studies are discussed. In\u00a0vitro investigations demonstrated an increase in the levels of OGT and O\u2010GlcNAcylation in nucleus pulposus cells (NPCs) following exposure to tert\u2010butyl hydroperoxide (TBHP). APS further facilitated improvements in OGT expression and O\u2010GlcNAcylation processes, restoring the viability of NPCs inhibited by TBHP and promoting the synthesis of collagen II and aggrecan, while reducing apoptosis. Mechanistically, APS promotes the expression of OGT by targeting it. Furthermore, O\u2010GlcNAcylation mediated by OGT stabilizes the expression of Nrf2 via the ubiquitin\u2010proteasome pathway. Rescue experiments indicated that the disruption of either OGT or Nrf2 expression negated the protective role of APS on NPCs. Ultimately, both in\u00a0vitro and in\u00a0vivo studies indicated that APS significantly enhanced OGT expression and O\u2010GlcNAcylation, which subsequently improved Nrf2 expression and contributed to the alleviation of IVDD in rats. APS promotes O\u2010GlcNAcylation through OGT, thereby stabilizing the expression of Nrf2, which in turn contributes to the improvement of IVDD.<\/jats:p>","DOI":"10.1111\/jcmm.70940","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T04:41:16Z","timestamp":1765428076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Astragalus Polysaccharide Enhances O\u2010\n                    <scp>GlcNAcylation<\/scp>\n                    Through\n                    <scp>OGT<\/scp>\n                    to Improve Intervertebral Disc Degeneration in Rats"],"prefix":"10.1111","volume":"29","author":[{"given":"Hao","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Orthopedics Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital)  Changsha 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