{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:13:20Z","timestamp":1784261600542,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["2023B1212060063"],"award-info":[{"award-number":["2023B1212060063"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82174285"],"award-info":[{"award-number":["82174285"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82305246"],"award-info":[{"award-number":["82305246"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A6012"],"award-info":[{"award-number":["U23A6012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515110720"],"award-info":[{"award-number":["2022A1515110720"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Psoriasis is a chronic, immune\u2010mediated skin disease. While redox imbalance has been implicated in its pathogenesis, the involvement of newly identified cell death pathways remains unclear. Disulfidptosis, a novel form of regulated cell death driven by intracellular disulfide stress, has recently been linked to inflammatory and metabolic diseases, yet its role in psoriasis is unknown. We conducted an integrative analysis combining bulk transcriptomic data (GSE106992 and GSE11239) and single\u2010cell RNA sequencing data (GSE162183) to identify disulfidptosis\u2010related genes (DRGs) associated with psoriasis. Differentially expressed genes were intersected with a curated DRG list. Feature selection was performed using LASSO regression and random forest algorithms, followed by WGCNA to identify key modules. Immune infiltration and cell\u2010type\u2010specific expression were assessed, and findings were validated using an imiquimod\u2010induced psoriasis\u2010like mouse model through Western blotting. To investigate the role of disulfidptosis in psoriasis, we integrated bulk and single\u2010cell RNA sequencing datasets from psoriatic lesional and non\u2010lesional skin. Differential expression analysis, WGCNA, and machine learning approaches (LASSO regression and random forest) were employed to identify DRGs. Immune cell infiltration patterns were analyzed, and gene expression was validated in an imiquimod\u2010induced psoriasis\u2010like mouse model using Western blotting. Seven DRGs were differentially expressed in psoriatic lesions. Machine learning approaches converged on five candidate genes, with GYS1, SLC3A2, FLNA, FLNB, and TLN1 showing strong discriminatory power (AUC &gt;\u20090.7). WGCNA identified four hub genes (GYS1, SLC3A2, TLN1, and FLNB) associated with disease\u2010relevant gene modules. Immune infiltration analysis revealed significant correlations between DRG expression and key immune subsets, including activated CD4\n                    <jats:sup>+<\/jats:sup>\n                    memory T cells and M1 macrophages. Single\u2010cell RNA\u2010seq confirmed cell type\u2013specific enrichment of DRGs in epidermal, mesenchymal, and immune cell populations. Protein\u2010level validation in the murine model further supported transcriptional upregulation of these genes in psoriatic lesions. Our findings suggest that disulfidptosis\u2010related pathways may contribute to psoriasis pathogenesis through interactions with immune infiltration. The identified hub genes, particularly GYS1 and SLC3A2, represent potential biomarkers and therapeutic targets, offering new insight into the complex molecular landscape of psoriasis.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70945","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T03:51:21Z","timestamp":1763005881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Disulfidptosis\u2010Associated Hub Genes in Psoriasis via Integrated Transcriptomic and Experimental Validation Approaches"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7611-8844","authenticated-orcid":false,"given":"Siyu","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), the Second Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taoyu","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), the Second Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyiming","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), the Second Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangqing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), the Second Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyun","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), the Second Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanjian","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), the Second Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macau Joint Lab on Chinese Medicine and Immune Disease Research Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"State Key Laboratory of Dampness Syndrome of Chinese Medicine The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine)  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maojie","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), the Second Clinical Medical College of Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macau Joint Lab on Chinese Medicine and Immune Disease Research Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"State Key Laboratory of Dampness Syndrome of Chinese Medicine The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine)  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome The Second Affiliated Hospital of Guangzhou University of Chinese Medicine  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