{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T05:48:52Z","timestamp":1773380932682,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000098","name":"Clinical Center","doi-asserted-by":"publisher","award":["R01 HD076422"],"award-info":[{"award-number":["R01 HD076422"]}],"id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000098","name":"Clinical Center","doi-asserted-by":"publisher","award":["HD052668"],"award-info":[{"award-number":["HD052668"]}],"id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Asherman Syndrome (AS) is caused by injury to the endometrium leading to uterine scarring, decreased menstruation and infertility; it typically occurs after surgical curettage of the uterus. AS is treated surgically albeit with limited success. Administration of bone marrow\u2010derived mesenchymal stem cells (MSCs) has recently been demonstrated to restore uterine function in AS; however, there is no data available on the role of the estrous cycle phase on outcomes. Here, we describe endometrial injury during estrus or diestrus, its differential effect on fertility, and its response after bone marrow MSC treatment to reverse the infertility in a murine model. Endometrial injury in the estrus phase did not affect fertility outcomes whereas injury in the diestrus phase resulted in infertility. Bone marrow (BM)\u2010derived MSC treatment without injury in the estrus or diestrus phase did not affect the pregnancy outcomes. BM MSC treatment following endometrial injury in the diestrus phase restored fertility. Immunofluorescence analysis revealed that vimentin or cytokeratin\u2010positive BM\u2010derived cells in the uterus were extremely rare. BM MSC treatment after injury increased CD45\n                    <jats:sup>+<\/jats:sup>\n                    cells, indicating a role for immunomodulation in endometrial repair. Finally, qRT\u2010PCR showed that\n                    <jats:italic>Ccl3<\/jats:italic>\n                    ,\n                    <jats:italic>Il\u20101\u03b2<\/jats:italic>\n                    and\n                    <jats:italic>Mmp3<\/jats:italic>\n                    gene expression was significantly higher in the endometrium of the injury + BM MSC group than in other groups. In summary, injury to the endometrium during the diestrus phase results in infertility that can be restored by the treatment of BM MSCs. The therapeutic effect of BM MSCs on the endometrium appears to be mediated primarily by immunomodulation rather than BM MSC engraftment.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70966","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T11:52:04Z","timestamp":1764157924000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Endometrial Injury and Its Rescue by Mesenchymal Stem Cells Is Dependent on Estrous Cycle Phase"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4022-8910","authenticated-orcid":false,"given":"Ramanaiah","family":"Mamillapalli","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynecology &amp; Reproductive Sciences Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology &amp; Reproductive Sciences Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuping","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology &amp; Reproductive Sciences Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reshef","family":"Tal","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology &amp; Reproductive Sciences Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugh S.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology &amp; Reproductive Sciences Yale School of Medicine  New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010015\u20100032\u20101"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032\u2010020\u201000279\u20103"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.04.043"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107827\u201011\u201066"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c10884"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659\u2010024\u201000484\u20103"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2016.0062"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/s096719941900042x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010018\u20100777\u20105"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-04454-7"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.292.1.81"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006\u20100828"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2016\u20101418"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.107987"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007\u20100301"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2011.0193"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13294"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.3431"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000421"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096662"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew042"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1808737"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2021.01.002"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2015.04.004"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719116682879"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2013\u20101977"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2010.10.007"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy175"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2017.01.001"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3712"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100897.2009.00792.x"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep\u201014\u20100395"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010024\u201003716\u20101"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/1598127"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu059"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103\u2010016\u20101923\u2010x"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910808700805"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204991109"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2125"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080705"},{"issue":"5","key":"e_1_2_11_42_1","first-page":"1706","article-title":"Down Regulation of MiR\u201093 Contributes to Endometriosis Through Targeting MMP3 and VEGFA","volume":"5","author":"Lv X.","year":"2015","journal-title":"American Journal of Cancer Research"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.70966","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T08:18:10Z","timestamp":1764404290000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.70966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":41,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/jcmm.70966"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.70966","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"2025-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70966"}}