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We examined the neurotoxic effects of Amx in zebrafish and investigated the potential of liposome\u2010encapsulated melatonin (L\u2010Mel) as a therapeutic intervention. Computational studies have indicated that Amx and Mel interact with GABA receptors, suggesting the potential of L\u2010Mel in mitigating Amx\u2010induced neurological changes. Our findings demonstrated that the nanoformulated L\u2010Mel showed reduced toxicity in zebrafish larvae. Administration of L\u2010Mel to Amx\u2010affected zebrafish brain tissue significantly lowered the levels of reactive oxygen species, antioxidants (catalase, superoxide dismutase, and nitric oxide), and proinflammatory cytokines (TNF\u2010\u03b1, IL\u20101\u03b2, and NF\u2010kB), based on the fixed EC\u201050. Behavioural assessments revealed that L\u2010Mel treatment notably enhanced the immobility time and swimming performance, improving the movement abilities of zebrafish with Amx\u2010induced neuroinflammation. Moreover, the GABA\/glutamate levels in the neural tissues exhibited significant recovery in the L\u2010Mel group. Gene and protein analysis showed substantial increases in BDNF, CREBBP, ASCL, NF\u2010\u03baB and GABA\u2010A R \u03b32 in L\u2010Mel treated subjects. Histopathological evaluation revealed that L\u2010Mel treatment markedly attenuated Amx\u2010induced neurotoxicity, as evidenced by reduced neuronal degeneration and necrosis in the brain tissue, indicating a pronounced neuroprotective effect. In conclusion, our research suggests that L\u2010Mel is a promising therapeutic agent for mitigating Amx\u2010induced neurotoxicity.<\/jats:p>","DOI":"10.1111\/jcmm.70969","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T11:52:46Z","timestamp":1764157966000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Liposome\u2010Encapsulated Melatonin Mitigates Amoxicillin\u2010Induced Neurotoxicity in a Zebrafish"],"prefix":"10.1111","volume":"29","author":[{"given":"Ranjith","family":"Balakrishnan","sequence":"first","affiliation":[{"name":"Centre for Advanced Biotherapeutics and Regenerative Medicine, Faculty of Research, Chettinad Hospital and Research Institute Chettinad Academy of Research and Education  Kelambakkam Tamil Nadu 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