{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:35:33Z","timestamp":1790660133088,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV\u201022\u20100271"],"award-info":[{"award-number":["APVV\u201022\u20100271"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["VEGA 2\/0131\/24"],"award-info":[{"award-number":["VEGA 2\/0131\/24"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Myocardial infarction (MI) is a sudden necrosis of cardiomyocytes, often caused by atherosclerosis, with obesity being a significant risk factor. This study aimed to evaluate the effects of a high\u2010fat diet (HFD) on post\u2010MI myocardial remodelling, focusing on inflammatory signalling, nitric oxide synthase (NOS) dysregulation and oxidative stress. Nine\u2010week\u2010old Wistar Kyoto rats fed a control diet or a HFD for 4\u2009weeks, followed by 20\u2009min of left descending coronary artery occlusion and 7\u2009days of reperfusion. Plasma levels of the proinflammatory cytokines TNF\u2010\u03b1 and IL\u20106 were measured using a Bioplex kit. NOS activity was assessed via [\n                    <jats:sup>3<\/jats:sup>\n                    H]\u2010\n                    <jats:sc>l<\/jats:sc>\n                    \u2010citrulline production, while the expression of eNOS, iNOS, NF\u03baB and TLR4 in cardiac tissue was analysed by Western blot. Lipid peroxidation was assessed by measuring conjugated diene concentrations in cardiac tissue. MI and HFD both increased proinflammatory cytokine levels, reduced NOS activity and eNOS expression and increased iNOS expression. NF\u03baB was more highly expressed after MI in control\u2010fed animals. Notably, TLR4 expression was reduced by HFD and remained unchanged post\u2010MI. Conjugated dienes were elevated post\u2010MI and further increased by HFD. These findings demonstrate that HFD exacerbates post\u2010MI inflammation and oxidative stress, impairing nitric oxide signalling and promoting adverse cardiac remodelling.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.70984","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:49:01Z","timestamp":1765597741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of High\u2010Fat Diet on Biochemical Changes Following Experimental Myocardial Infarction"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9421-264X","authenticated-orcid":false,"given":"Katarina","family":"Bujnova","sequence":"first","affiliation":[{"name":"Department of Neuro\u2010Cardiovascular Interaction, Institute of Normal and Pathological Physiology Centre of Experimental Medicine, Slovak Academy of Sciences  Bratislava 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