{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:42:16Z","timestamp":1790703736367,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2024JJ6341"],"award-info":[{"award-number":["2024JJ6341"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82505793"],"award-info":[{"award-number":["82505793"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Between 50% and 80% of children diagnosed with Autism Spectrum Disorder (ASD) are estimated to experience sleep disturbances, highlighting the importance of exploring the role of the circadian clock in ASD development. Previous studies have identified a potential link between Bmal1 deficiency and ASD in mouse models. In this study, we first characterise the expression patterns of circadian proteins. Subsequent behavioural tests and western blot analyses revealed that mice exposed to valproic acid (VPA) displayed autistic\u2010like behaviours, along with altered circadian protein expression and disruption in Wnt signalling protein levels. Further studies showed that Bmal1 knockout exacerbates these behavioural changes and further impaired Wnt signalling and downstream protein expression in VPA\u2010exposed mice. Notably, treatment with the circadian biomarker melatonin reversed Wnt downregulation and improved the behaviour deficit in VPA\u2010exposed mice. The therapeutic effect of melatonin appears to be mediated by its regulation of the Wnt\/\u03b2\u2010catenin signalling pathway, which is linked to Bmal1\u2010mediated circadian dysfunction. Together, our findings provide experimental evidence supporting the role of circadian dysregulation in ASD pathogenesis, highlight the therapeutic potential of melatonin in VPA\u2010exposed mice, and suggest that Bmal1 may act as a co\u2010activator in the Wnt\u2010\u03b2\u2010catenin signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.70991","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:04:13Z","timestamp":1767845053000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Circadian Clock Dysfunction Exacerbate Autistic\u2010Like Behaviour and Wnt\/\u03b2\u2010Catenin Signalling Dysregulation in ASD Mice and Treatment of Melatonin"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8515-7861","authenticated-orcid":false,"given":"Yuxing","family":"Zhang","sequence":"first","affiliation":[{"name":"Hunan University of Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinan","family":"Chen","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine  Changsha Hunan Province China"},{"name":"Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio\u2010Cerebral Diseases  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liya","family":"Tang","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangshan","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3591-2435","authenticated-orcid":false,"given":"Xiang","family":"Feng","sequence":"additional","affiliation":[{"name":"Hunan University of Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"issue":"10146","key":"e_1_2_14_2_1","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/S0140-6736(18)31129-2","article-title":"Autism Spectrum Disorder","volume":"392","author":"Lord C.","year":"2018","journal-title":"Lancet"},{"key":"e_1_2_14_3_1","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.2147\/NDT.S331985","article-title":"Detecting Time Concept Competence in Children With Autism Spectrum and Attention Disorders","volume":"18","author":"Hus Y.","year":"2022","journal-title":"Neuropsychiatric Disease and Treatment"},{"issue":"1","key":"e_1_2_14_4_1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.chc.2020.08.012","article-title":"Autism Spectrum Disorder and Sleep","volume":"30","author":"Johnson K. 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