{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:13:29Z","timestamp":1791461609486,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Tripartite motif 52 (TRIM52) has been identified as a key regulator of inflammatory responses. However, its involvement in doxorubicin (DOX)\u2010induced cardiotoxicity (DIC) and the underlying molecular mechanisms remain poorly understood. To investigate the functional role of TRIM52, we employed an adeno\u2010associated virus serotype 9 (AAV9) delivery system to achieve cardiac\u2010specific Trim52 knockout via tail\u2010vein injection. C57BL\/6 mice received intraperitoneal DOX (5\u2009mg\/kg, administered once a week, with a total cumulative dose of 15\u2009mg\/kg). Myocardial injury was evaluated by histopathological assessment and molecular profiling of cardiac tissues, complemented by in\u00a0vitro mechanistic studies using neonatal mouse cardiomyocytes. In\u00a0vivo and in\u00a0vitro studies revealed that DOX treatment significantly upregulated TRIM52 expression. Trim52 deficiency effectively mitigated DOX\u2010induced cardiac injury and dysfunction, concomitantly attenuating oxidative stress and inflammatory responses. Mechanistically, Trim52 deletion markedly enhanced PI3K and AKT phosphorylation, indicating that PI3K\/AKT pathway activation underlies the cardioprotective effects of TRIM52 deficiency. Our findings demonstrate that TRIM52 deletion activates PI3K\/AKT signalling and attenuates DOX\u2010induced oxidative and inflammatory myocardial damage. These data identify TRIM52 as a potential therapeutic target for mitigating DIC.<\/jats:p>","DOI":"10.1111\/jcmm.71016","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T10:50:52Z","timestamp":1767955852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>TRIM52<\/scp>\n                    Protects Against Doxorubicin\u2010Induced Cardiac Inflammation, Oxidative Stress and Cardiac Injury"],"prefix":"10.1111","volume":"30","author":[{"given":"Zhaoxia","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Ningbo University, The Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Ningbo University, The Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchu","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Ningbo University, The Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiedong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Health Science Center Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqi","family":"Lu","sequence":"additional","affiliation":[{"name":"Health Science Center Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingsha","family":"Du","sequence":"additional","affiliation":[{"name":"Health Science Center Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Jia","sequence":"additional","affiliation":[{"name":"Health Science Center Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Ningbo University, The Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5484-8744","authenticated-orcid":false,"given":"Weiping","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Ningbo University, The Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.7150\/ijbs.65258","article-title":"Underlying the Mechanisms of Doxorubicin\u2010Induced Acute Cardiotoxicity: Oxidative Stress and Cell Death","volume":"18","author":"Kong C. 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