{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:45:00Z","timestamp":1785498300394,"version":"3.56.0"},"reference-count":99,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2026NSFSC0492;2026NSFSC1819"],"award-info":[{"award-number":["2026NSFSC0492;2026NSFSC1819"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Periodontitis is one of the most common oral inflammatory diseases. Baiyaojian decoction, known for its prominent immunomodulatory and anti\u2010inflammatory properties, shows significant potential in treating periodontitis, though its molecular mechanisms remain unknown. The active ingredients and therapeutic targets were determined by integrating multiple databases. The protein\u2013protein interaction network was constructed by the STRING platform. Bulk RNA seq data of GSE16134 were included and GO enrichment, GSEA and CIBERSORT algorithm were employed to investigate the immune microenvironment in periodontitis. Single\u2010cell RNA seq data of GSE152042 and GSE171213 were integrated by harmony; the cell\u2013cell communication network was analysed by CellChat, and the differentiation trajectory was constructed by monocle3. Molecular docking was carried out using AutoDockTools, AutoDock Vina and PyMOL. Experimental validation was performed via qRT\u2010PCR, CCK\u20108 assay, flow cytometry and ELISA. Twenty\u2010seven active ingredients and 207 therapeutic targets were obtained. Thirty\u2010one core therapeutic targets were identified. The infiltration of plasma cells, neutrophils, macrophages and mast cells was significantly enhanced in periodontitis tissues. Twenty\u2010eight of 31 core therapeutic targets were involved in their infiltration, differentiation and pro\u2010inflammatory activities. Molecular docking suggested stable bindings between ingredients and therapeutic targets. Experimental validation confirmed the elevated infiltration of above immune cells and demonstrated the anti\u2010inflammatory properties and target modulation capabilities of key ingredients including Coumestrol, Diosmetin and Gallicin. Baiyaojian decoction may exert immunomodulatory and anti\u2010inflammatory effects to treat periodontitis through multi\u2010ingredient and multi\u2010target mechanisms.<\/jats:p>","DOI":"10.1111\/jcmm.71034","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:00:27Z","timestamp":1769659227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunomodulatory Mechanism of Baiyaojian Decoction on Periodontitis: Network Pharmacology, Single\u2010Cell\n                    <scp>RNA<\/scp>\n                    Sequencing and Molecular Docking"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7491-4628","authenticated-orcid":false,"given":"Bing\u2010jun","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Stomatology Hospital of Chengdu University of Traditional Chinese Medicine  Chengdu People's Republic of 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