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Mixed lineage kinase domain\u2010like protein (\n                    <jats:italic>MLKL<\/jats:italic>\n                    )\u2010mediated necroptosis, an essential form of regulated cell death implicated in various pulmonary disorders, has not been fully investigated in the context of BPD. Here, we utilised a neonatal mouse model of hyperoxia exposure to elucidate the role and mechanisms of\n                    <jats:italic>MLKL<\/jats:italic>\n                    \u2010mediated necroptosis in BPD pathogenesis. Our analysis demonstrated morphological characteristics of necroptosis in pulmonary vascular endothelial cells (ECs) under hyperoxic conditions, accompanied by significant elevation of\n                    <jats:italic>MLKL<\/jats:italic>\n                    protein levels and marked upregulation of\n                    <jats:italic>MLKL<\/jats:italic>\n                    gene expression specifically in vascular ECs. Administration of the\n                    <jats:italic>MLKL<\/jats:italic>\n                    inhibitor necrosulfonamide (NSA), either immediately postnatally or at postnatal day 7, effectively mitigated lung injury, preserved alveolar structure and partially restored pulmonary vascular growth. Moreover,\n                    <jats:italic>MLKL<\/jats:italic>\n                    conditional knockout in ECs significantly attenuated both structural and functional pulmonary abnormalities induced by hyperoxia. Collectively, our findings indicate that\n                    <jats:italic>MLKL<\/jats:italic>\n                    \u2010mediated necroptosis in vascular ECs plays a pivotal role in hyperoxia\u2010induced BPD. Therapeutically targeting\n                    <jats:italic>MLKL<\/jats:italic>\n                    to maintain endothelial integrity presents a promising approach to prevent or alleviate BPD in premature infants.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71035","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T10:28:12Z","timestamp":1769682492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Endothelial\n                    <scp>\n                      <i>MLKL<\/i>\n                    <\/scp>\n                    Inhibition Reduces Hyperoxia\u2010Induced Bronchopulmonary Dysplasia in Neonatal Mice"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8555-5480","authenticated-orcid":false,"given":"Junjie","family":"Ning","sequence":"first","affiliation":[{"name":"Department of Pediatrics, West China Second University Hospital Sichuan University  Chengdu China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junchao","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yating","family":"Sang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, West China Second University Hospital Sichuan University  Chengdu China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2866-2124","authenticated-orcid":false,"given":"Lina","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, West China Second University Hospital Sichuan University  Chengdu China"},{"name":"Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education Sichuan University  Chengdu China"},{"name":"NHC Key Laboratory of Chronobiology Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196702162760701"},{"key":"e_1_2_12_3_1","article-title":"Diagnosis and Management of Bronchopulmonary Dysplasia","volume":"375","author":"Gilfillan M.","year":"2021","journal-title":"BMJ (Clinical Research 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