{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:47:13Z","timestamp":1792061233292,"version":"4.4.0"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AR084530"],"award-info":[{"award-number":["R21AR084530"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD055887"],"award-info":[{"award-number":["HD055887"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007249","name":"University of Minnesota","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007249","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Although studies support disrupted bone remodelling within geriatric populations, mid\u2010life changes are understudied. To investigate this, we performed bone marrow transplantation assays using either 8\u2010 or 40\u2010week\u2010old mice. Micro\u2010CT analyses of lethally irradiated 8\u2010week\u2010old mice transplanted with 40\u2010week\u2010old bone marrow exhibited increased mid\u2010shaft femoral cortical bone mass and thickness. Intensive bone marrow regeneration mirrors hematopoietic development in that erythro\u2010myeloid progenitors (EMPs) first expand to support blood production before definitive hematopoietic stem cell (HSC) production. We hypothesized that reduced HSC capacity of 40\u2010week\u2010old bone marrow and compensatory expansion of EMPs may facilitate gains in cortical bone. Flow cytometry analyses revealed greater EMP to HSC ratios when mice were reconstituted with increasing percentages of middle\u2010aged bone marrow. To identify cell types mediating these effects, we performed comparative scRNA\u2010Seq analyses and identified CD11B\n                    <jats:sup>+<\/jats:sup>\n                    CD36\n                    <jats:sup>+<\/jats:sup>\n                    myeloid cells exhibiting enriched expression of bone anabolic cytokines. Elevated levels of Wnt ligands, especially Wnt6, characterized these cells. In lineage tracing assays, CD11B\n                    <jats:sup>+<\/jats:sup>\n                    CD36\n                    <jats:sup>+<\/jats:sup>\n                    cells were donor\u2010derived myeloid cells. In functional assays, we demonstrate that soluble factors produced by CD11B\n                    <jats:sup>+<\/jats:sup>\n                    CD36\n                    <jats:sup>+<\/jats:sup>\n                    cells enhance osteogenesis. Moreover, CD11B\/CD36\/Wnt6 exquisitely mark anabolic macrophages within human bone marrow. These findings reveal a myeloid population present during midlife that enhances cortical bone.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71094","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T05:32:51Z","timestamp":1774330371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Old Blood, Young Bones: Identification of Middle\u2010Aged Myeloid Cells That Limit Cortical Bone Loss"],"prefix":"10.1111","volume":"30","author":[{"given":"Jinsha","family":"Koroth","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, Medical School University of Minnesota  Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismael Y.","family":"Karkache","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Medical School University of Minnesota  Minnesota USA"},{"name":"College of Veterinary Sciences University of Minnesota  Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth K.","family":"Vu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Medical School University of Minnesota  Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph C.","family":"Manser","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Medical School University of Minnesota  Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchel J.","family":"Shimak","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Medical School University of Minnesota  Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim C.","family":"Mansky","sequence":"additional","affiliation":[{"name":"Division of Orthodontics, School of Dentistry University of Minnesota  Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8814-5524","authenticated-orcid":false,"given":"Elizabeth W.","family":"Bradley","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Medical School University of Minnesota  Minnesota USA"},{"name":"Stem Cell Institute University of Minnesota  Minnesota 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