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Dysfunction of bone marrow mesenchymal stem cells (BMSCs) and their exosome\u2010mediated signalling is a key contributor to SONFH, although the precise mechanisms remain to be elucidated. In this study, the differential expression profiles of long noncoding RNAs (lncRNAs), microRNAs (miRNAs) and messenger RNAs (mRNAs) in exosomes derived from human BMSCs (hBMSCs) obtained from patients with SONFH compared to controls with femoral neck fractures were identified. Through next\u2010generation sequencing, a novel lncRNA, LNC000133, associated with SONFH was discovered. Using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and competing endogenous RNA (ceRNA) network construction, the LNC000133\/miR\u2010362\u20105p\/TGF\u2010\u03b23\/SMAD3\/BMP2 signalling axis was established. The definitive expression, localization and full\u2010length sequence of LNC000133 in BMSCs were subsequently validated by Northern blot, quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR), fluorescence in\u00a0situ hybridization (FISH) and rapid amplification of cDNA ends (RACE). Most notably, mechanistic studies demonstrated that LNC000133\u2010modified BMSCs\u2010derived exosomes were efficiently taken up by osteoblasts, which promoted proliferation and osteogenic differentiation by targeting the miR\u2010362\u20105p\/TGF\u2010\u03b23\/SMAD3\/BMP2 signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.71135","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T03:51:12Z","timestamp":1776484272000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Novel Long Noncoding\n                    <scp>RNA<\/scp>\n                    \u2013\n                    <scp>LNC000133<\/scp>\n                    Associated With Steroid\u2010Induced Osteonecrosis of the Femoral Head Promotes Osteoblast Differentiation Through Bone Marrow Mesenchymal Stem Cells\u2010Derived Exosomes Pathway: A Bioinformatics Validation and Detailed Mechanistic 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