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It is now recognized that both maternal and fetal cells contribute to disease progression. Notably, single\u2010cell RNA sequencing of cord blood cells from preeclamptic pregnancies has not been previously investigated, and this became the focus of our study. In this work, we performed flow cytometry, single\u2010cell RNA sequencing and bioinformatics analysis of cord blood mononuclear cells from preeclampsia and control groups. Bulk RNA\u2010sequencing data of preeclamptic cord blood from open source was also analysed. We observed a significant decrease in the expression of the CD3, CD8 and CD4 markers in cord blood mononuclear cells in the preeclampsia group. Single\u2010cell RNA sequencing revealed activation of iron\u2010 and heme\u2010associated signalling pathways in various cell types (nonclassical monocytes, naive \u0421D4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, T\u2010helpers 2, NK cells and naive \u0421D4\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T cells) in cord blood during preeclampsia. Analysis of open\u2010source bulk RNA\u2010sequencing data confirmed our findings of activation of heme\u2010related signalling pathways in preeclamptic cord blood samples. These results may suggest the activation of compensatory mechanisms, potentially mediated by the proangiogenic heme degradation products carbon monoxide and biliverdin. Additionally, free heme may act as a proinflammatory damage\u2010associated molecular pattern in preeclampsia. Further studies are needed to elucidate the direct relationship between these mechanisms and placental heme metabolism.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71150","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:20:58Z","timestamp":1777436458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Analysis of Preeclamptic Cord Blood Mononuclear Cells Revealed Activation of Heme\u2010Associated Signalling Pathways"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8650-8240","authenticated-orcid":false,"given":"Polina","family":"Vishnyakova","sequence":"first","affiliation":[{"name":"National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. 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