{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:54:13Z","timestamp":1781535253778,"version":"3.54.5"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2024AFD318"],"award-info":[{"award-number":["2024AFD318"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to elucidate the mechanism of action of Schisandrin A in the intervention of triple\u2010negative breast cancer (TNBC). Through the application of network pharmacology and the integration of targets from multiple databases, the research identified 128 overlapping targets between Schisandrin A and TNBC. From these, 36 core targets were determined via topological analysis. Utilizing the random forest survival algorithm, four prognosis\u2010related core targets (GSK3B, IDO1, KDR, PKM) were identified from the METABRIC database, and the model's predictive performance was validated using the GSE58812 dataset. Pan\u2010cancer expression analysis confirmed the aberrant expression of these four targets across various tumours. Immune infiltration analysis suggested that GSK3B and IDO1 may influence the tumour immune microenvironment. Molecular docking studies demonstrated a high binding affinity of Schisandrin A with IDO1 and PKM. In\u00a0vitro cell experiments indicated that Schisandrin A inhibited the proliferation of MDA\u2010MB\u2010231 cells in a concentration\u2010dependent manner, induced apoptosis and caused cell cycle arrest at the S phase. Transcriptome sequencing further revealed the transcriptional changes induced by Schisandrin A, elucidating that the cell cycle and DNA replication are the primary regulatory pathways affected. In conclusion, Schisandrin A exhibits potential anti\u2010triple\u2010negative breast cancer (TNBC) effects by modulating and regulating diverse pathways, including GSK3B and IDO1, alongside its impact on the cell cycle and immune microenvironment. This study presents novel candidate drugs and therapeutic targets for the precise treatment of TNBC.<\/jats:p>","DOI":"10.1111\/jcmm.71219","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T07:34:43Z","timestamp":1780299283000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Network Pharmacology and Machine Learning to Reveal the Mechanisms of Schisandrin A Against Triple\u2010Negative Breast Cancer"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5836-6891","authenticated-orcid":false,"given":"Meng","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Maternal and Child Health Hospital of Hubei Province  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese 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