{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:54:12Z","timestamp":1781535252694,"version":"3.54.5"},"reference-count":58,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colorectal cancer is a prevalent malignancy, with colon adenocarcinoma (COAD) representing its most common histological subtype. Although most stage I patients remain disease\u2010free after treatment, a subset rapidly progresses to advanced disease with markedly reduced survival. Therefore, identifying early molecular warning signals in stage I patients is essential for timely intervention. In this study, integrated datasets from The Cancer Genome Atlas and Genotype\u2010Tissue Expression projects were analysed using the landscape dynamic network biomarker (\n                    <jats:italic>l<\/jats:italic>\n                    \u2010DNB) approach to identify progression biomarkers (PBs) for COAD. Twenty PBs were identified, among which succinate dehydrogenase complex flavoprotein subunit A (SDHA) was selected for further investigation. The L1000CDS\n                    <jats:sup>2<\/jats:sup>\n                    database was subsequently queried utilizing these identified PBs, identifying Cucurbitacin I (CuI) as a promising therapeutic component against COAD. Molecular docking and molecular dynamics simulations demonstrated stable binding between CuI and SDHA, with a binding energy of \u22129.44\u2009kcal\/mol and an RMSD of 2.0\u2009\u00b1\u20090.3\u2009\u00c5. Subsequent in\u00a0vitro experiments demonstrated that CuI treatment upregulated SDHA expression and inhibited activation of the NF\u2010\u03baB pathway. Collectively, these findings suggest that the identified PBs may serve as early\u2010warning indicators for stage I COAD patients and that CuI suppresses COAD cell proliferation, potentially through the SDHA\/NF\u2010\u03baB axis, highlighting its promise as a potential therapeutic candidate.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71231","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T03:55:56Z","timestamp":1781063756000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of Cucurbitacin I in Colon Adenocarcinoma Is Mediated by Modulation of\n                    <scp>SDHA<\/scp>\n                    Expression"],"prefix":"10.1111","volume":"30","author":[{"given":"Danni","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Intelligent Medicine Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunzhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenke","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy Chengdu University of Traditional Chinese Medicine  Chengdu China"},{"name":"Institute of Herbgenomics Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Intelligent Medicine Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengmian","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Innovative Institute of Chinese Medicine and Pharmacy Chengdu University of Traditional Chinese Medicine  Chengdu China"},{"name":"Institute of Herbgenomics Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"issue":"3","key":"e_1_2_13_4_1","first-page":"153","article-title":"Colorectal Carcinoma: Pathologic Aspects [J]","volume":"3","author":"Fleming M.","year":"2012","journal-title":"J Gastrointest Oncol"},{"issue":"9","key":"e_1_2_13_5_1","first-page":"1059","article-title":"Histological Grading in Colorectal Cancer: New Insights and Perspectives [J]","volume":"30","author":"Barresi V.","year":"2015","journal-title":"Histology and Histopathology"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21601"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21654"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61649-9"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00342"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwy162"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.06.023"},{"issue":"2","key":"e_1_2_13_12_1","first-page":"304","article-title":"Identifying Critical State of Breast Cancer Cell Differentiation Based on Landscape Dynamic Network Biomarkers","volume":"37","author":"Zhao H. 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