{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:54:12Z","timestamp":1781535252082,"version":"3.54.5"},"reference-count":68,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Colorectal cancer (CRC) is one of the most common malignancies worldwide and remains a major cause of cancer\u2010related mortality. Increasing evidence suggests that aberrant RNA metabolism contributes to tumour initiation, progression and therapeutic resistance. In this study, we integrated single\u2010cell RNA sequencing and bulk transcriptomic data to identify RNA metabolism\u2010related genes (RMRGs) associated with CRC progression and evaluate their clinical significance. Through comprehensive bioinformatics analyses, 39 differentially expressed RMRGs were identified, with high RMRG activity predominantly enriched in epithelial cell populations. Cell\u2013cell communication analysis revealed enhanced interactions between epithelial cells and other cell types within the tumour microenvironment. Further integration of single\u2010cell and bulk RNA\u2010sequencing datasets identified PCBP3 and NGRN as key prognostic genes. A two\u2010gene prognostic model based on PCBP3 and NGRN was established and validated in independent cohorts, demonstrating favourable predictive performance. Human Protein Atlas data further confirmed the expression of both proteins in colorectal cancer tissues. Immune infiltration analyses indicated that PCBP3 and NGRN were associated with distinct immune\u2010cell infiltration patterns and immunotherapy\u2010related immune status. Functional experiments demonstrated that silencing PCBP3 or NGRN significantly inhibited the proliferation and invasion of HCT116 colorectal cancer cells. Moreover, Western blot analysis suggested that these effects may be mediated, at least in part, through regulation of the PI3K\/AKT signalling pathway. Collectively, our findings identify PCBP3 and NGRN as promising prognostic biomarkers and potential therapeutic targets in colorectal cancer and provide new insights into the role of RNA metabolism in colorectal cancer progression and tumour immune regulation.<\/jats:p>","DOI":"10.1111\/jcmm.71236","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T04:00:13Z","timestamp":1781150413000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>RNA<\/scp>\n                    Metabolism Genes as Prognostic Biomarkers and Therapeutic Targets in Colorectal Cancer Based on the Analysis of Single\u2010Cell and Bulk\u2010\n                    <scp>RNA<\/scp>\n                    Sequencing Data"],"prefix":"10.1111","volume":"30","author":[{"given":"Fandong","family":"Kong","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Panyu Maternal and Child Care Service Centre of Guangzhou (He Xian Memorial Affiliated Hospital of Southern Medical University)  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuewei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery, Guangzhou First People's Hospital Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuguang","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pathology Panyu Maternal and Child Care Service Centre of Guangzhou (He Xian Memorial Affiliated Hospital of Southern Medical University)  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feilong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology Panyu Maternal and Child Care Service Centre of Guangzhou (He Xian Memorial Affiliated Hospital of Southern Medical University)  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiantao","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pathology Panyu Maternal and Child Care Service Centre of Guangzhou (He Xian Memorial Affiliated Hospital of Southern Medical University)  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9685-7003","authenticated-orcid":false,"given":"Ronghua","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery, Guangzhou First People's Hospital Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanpeng","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Panyu Maternal and Child Care Service Centre of Guangzhou (He Xian Memorial Affiliated Hospital of Southern Medical University)  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00615"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/cm9.0000000000003253"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/jom\u20102024\u20100026"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v16.i7.3032"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/almed\u20102024\u20100146"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010024\u201002108\u20106"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262\u2010024\u201003658\u2010w"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz\u20102023\u20100233"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2024.102097"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201062913\u20103"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/cm9.0000000000003113"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.34133\/research.0364"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1829"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2023.1297093"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010024\u201003132\u20106"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202302240"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.113327"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010024\u201003006\u20103"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.216672"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201070422\u20106"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.217062"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.24211"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.111938"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1193"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.11.005"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.Can\u201014\u20101592"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010022\u201002677\u2010z"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100619\u20100"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422\u2010019\u20100195\u2010y"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.177428"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021246\u20109"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1038"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.08.038"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.02.052"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.26476"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08822"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2022.02.001"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575\u2010023\u201000841\u20109"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/procel\/pwad038"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.97975"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010024\u201002089\u20106"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.01.019"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001734\u20102"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.03.021"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2024.109870"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113610"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13607"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellin.2024.100170"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.08.040"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148\u2010024\u201001736\u20105"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41440\u2010024\u201001607\u20109"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931\u2010023\u201002606\u2010z"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010024\u201006606\u20109"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.2337\/db23\u20100360"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418\u2010023\u201001247\u20105"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2024.08.173"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.155153"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25084538"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2024.109375"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13293\u2010024\u201000630\u20102"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.34133\/research.0549"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.34133\/research.0488"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920\u2010024\u201001881\u2010z"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876\u2010023\u201003035\u20104"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.2147\/pgpm.S458798"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13030245"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.71236","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.71236","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.71236","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:21:12Z","timestamp":1781533272000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.71236"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":68,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/jcmm.71236"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.71236","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71236"}}