{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:54:09Z","timestamp":1781535249176,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["17\/15175\u20101"],"award-info":[{"award-number":["17\/15175\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["18\/09765\u20103"],"award-info":[{"award-number":["18\/09765\u20103"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["18\/05956\u20109"],"award-info":[{"award-number":["18\/05956\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["24\/03517\u20109"],"award-info":[{"award-number":["24\/03517\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Interstitial Cystitis\/Bladder Pain Syndrome (IC\/BPS) is a chronic condition characterized by bladder\/pelvic pain and urinary symptoms, with poorly defined mechanisms and no reliable biomarkers. Oxidative stress, particularly reactive oxygen species (ROS), has been implicated in its pathophysiology, but direct human evidence remains scarce. Here, we evaluated ROS\u2010 and nitric oxide (NO)\u2013related pathways in bladder mucosa from women with IC\/BPS (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) and controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20097). Gene expression of NO\u2013cGMP components, NADPH oxidases (NOX1\u20135), and antioxidant enzymes was assessed by qPCR. Baseline NO and superoxide () levels were quantified using histochemical assays, and the effects of pharmacological inhibitors of NOS, iNOS and NOX enzymes were examined. IC\/BPS samples displayed increased expression of PDE5A (3.1\u2010fold) and p47phox (1.6\u2010fold), along with reduced SOD1 expression (0.39\u2010fold), consistent with an oxidative imbalance. Although baseline  did not differ between groups, NOS inhibition with L\u2010NAME markedly reduced  generation, with a greater effect in IC\/BPS tissue (85% reduction). Conversely, selective iNOS inhibition (1400\u2009W) increased  levels in IC\/BPS biopsies. These findings suggest altered NOS\u2010dependent redox regulation in the IC\/BPS mucosa. Despite the limited sample size inherent to human biopsy studies, these results provide preliminary evidence of redox dysregulation in IC\/BPS mucosa and highlight  as a relevant mediator. They support further investigation into therapeutic strategies targeting NOS redox balance and cGMP signalling.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.71245","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T04:07:05Z","timestamp":1781237225000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Redox Imbalance and\n                    <scp>NOS<\/scp>\n                    \u2010Dependent Modulation of Superoxide in the Bladder Mucosa of Women With\n                    <scp>IC<\/scp>\n                    \/\n                    <scp>BPS<\/scp>\n                    : A Preliminary Study"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2226-2530","authenticated-orcid":false,"given":"Mariana G.","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology S\u00e3o Francisco University (USF)  Sao Paulo Brazil"},{"name":"Department of Pharmacology, Faculty of Medical Sciences University of Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2201-8247","authenticated-orcid":false,"given":"Edson","family":"Antunes","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences University of Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Reolon","family":"Passos","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences University of Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Gustavo Oliveira","family":"Brito","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Campinas Faculty of Medical Sciences  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Arturo Levi","family":"D'Ancona","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medical Sciences University of Campinas  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fab\u00edola Z.","family":"Monica","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medical Sciences University of Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585\u2010025\u201001078\u20108"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585\u2010023\u201000850\u2010y"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000546901"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12010092"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00041.2016"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2017.181"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.906818"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16045"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1551533"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201010\u2010199"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2008.03.184"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658\u2010023\u201000423\u20102"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1511529"}],"container-title":["Journal 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